Rothman R B, Herkenham M, Pert C B, Liang T, Cascieri M A
Brain Res. 1984 Aug 20;309(1):47-54. doi: 10.1016/0006-8993(84)91009-6.
Biochemical analysis of the binding of [125I]Bolton-Hunter coupled substance P [( 125I]BH-SP) to slide-mounted sections of rat brain demonstrated that [125I]BH-SP labels a binding site with a structure-activity profile characteristic of a substance P receptor. Under optimized preincubation and incubation conditions, the locations of substance P (SP) receptors were visualized by film and emulsion autoradiography. Receptor densities were quantified by computer-assisted densitometry. SP receptors are widely but discretely distributed throughout sensory, limbic and cortical areas of rat brain, though several motor areas also possess SP receptors. No receptors were detected in the substantia nigra and interpeduncular nucleus, which are innervated by SPergic nerves; these regions of the brain may possess a low affinity SP receptor not detectable with this assay. Findings are discussed in the framework of an overall notion of the role of neuropeptides in the biochemistry of emotion.
对[125I]博尔顿-亨特偶联P物质（[125I]BH-SP）与大鼠脑冰冻切片结合的生化分析表明，[125I]BH-SP标记的结合位点具有P物质受体的结构活性特征。在优化的预孵育和孵育条件下，通过放射自显影片和乳胶放射自显影观察P物质（SP）受体的位置。通过计算机辅助密度测定法定量受体密度。SP受体广泛但离散地分布于大鼠脑的感觉、边缘和皮质区域，尽管一些运动区域也有SP受体。在由P物质能神经支配的黑质和脚间核中未检测到受体；脑的这些区域可能具有用该检测方法无法检测到的低亲和力SP受体。研究结果在神经肽在情绪生物化学中作用的整体概念框架内进行了讨论。
