Beltrame F, Bianco B, Chiabrera A
Cell Biophys. 1984 Jun;6(2):103-16. doi: 10.1007/BF02788590.
A simplified theory of image formation in phase contrast microscopy is presented. It is shown that the phase shift induced in light (related to the refractive index) by the observed object can be reconstructed, point by point, from the phase-contrast digitally sampled image through an appropriate algorithm. This allows one to make quantitative observations on unstained, living cells.
本文提出了一种相衬显微镜成像的简化理论。研究表明，通过适当的算法，可以从相衬数字采样图像逐点重建被观察物体在光中引起的相移（与折射率相关）。这使得人们能够对未染色的活细胞进行定量观察。
