McKenna R M, Carter B G, Sehon A H
Cell Immunol. 1984 Oct 15;88(2):251-9. doi: 10.1016/0008-8749(84)90159-x.
The mechanism of suppression of experimental allergic encephalomyelitis (EAE) induced in Lewis rats by pretreatment with myelin basic protein (MBP) coupled to syngeneic spleen leukocytes (SL) was examined. Studies on the kinetics of the tolerance induction showed that pretreatment with MBP-SL suppressed EAE if given 7 but not 3 days before the disease-inducing injection of MBP in Freund's complete adjuvant. Treatment with cyclophosphamide 48 hr before administration of MBP-SL completely abolished the suppression of EAE. Transfer of lymph node and spleen cells from MBP-syngeneic erythrocyte conjugate (MBP-RBC) but not MBP-SL-pretreated rats resulted in suppression of disease in recipients subsequently given a disease-inducing injection of MBP. Administration of MBP coupled to SL from the histocompatible rat strain F344 resulted in suppression of the MBP-induced proliferative response of spleen cells from Lewis rats which had been given a disease-inducing injection of MBP. Taken together these results are consistent with the suppression of EAE induced by MBP-SL being mediated by suppressor T cells.
研究了用与同基因脾白细胞（SL）偶联的髓鞘碱性蛋白（MBP）预处理对Lewis大鼠诱导的实验性变应性脑脊髓炎（EAE）的抑制机制。对耐受性诱导动力学的研究表明，如果在弗氏完全佐剂中注射致病变应原性MBP前7天而非3天给予MBP-SL预处理，则可抑制EAE。在给予MBP-SL前48小时用环磷酰胺治疗可完全消除对EAE的抑制作用。来自MBP-同基因红细胞结合物（MBP-RBC）而非MBP-SL预处理大鼠的淋巴结和脾细胞转移，可抑制随后接受致病变应原性MBP注射的受体中的疾病。给予来自组织相容性大鼠品系F344的与SL偶联的MBP，可抑制已接受致病变应原性MBP注射的Lewis大鼠脾细胞的MBP诱导的增殖反应。综合这些结果表明，MBP-SL诱导的EAE抑制作用是由抑制性T细胞介导的。
