Gibson J C, Rubinstein A, Brown W V
Clin Chem. 1984 Nov;30(11):1784-8.
We measured the solubility of apolipoprotein E (apo E) after precipitation, with heparin-Mn2+ or dextran sulfate-Mg2+, of lipoproteins containing apo B. Data from 46 randomly selected subjects suggest that apo E is readily precipitated by dextran sulfate-Mg2+, but that heparin-Mn2+ preferentially precipitates apo E associated with apo B-containing lipoproteins while leaving the apo E-containing fraction of high-density lipoproteins (HDL) in solution. In a more detailed analysis of three subjects, we measured the lipoprotein association of apo E by column chromatography on agarose beads, before and after its precipitation from plasma. This study confirmed the preferential solubility of apo E associated with HDL lipoproteins. Using plasma from two normolipidemic subjects, we maintained the heparin concentration at 1.30 g/L and varied the manganese concentration from 9.2 to 184 mmol/L. A 46 mmol/L concentration best separated apo E-containing HDL from apo B-containing lipoproteins. Thus, at these final concentrations, heparin-Mn2+ appears to precipitate the apo E associated with apo B-containing lipoproteins, leaving soluble most of the apo E associated with lipoproteins of HDL size.
我们测定了用肝素 - 锰离子（heparin - Mn²⁺）或硫酸葡聚糖 - 镁离子（dextran sulfate - Mg²⁺）沉淀含载脂蛋白B（apo B）的脂蛋白后载脂蛋白E（apo E）的溶解度。来自46名随机选取受试者的数据表明，硫酸葡聚糖 - 镁离子能轻易沉淀apo E，但肝素 - 锰离子优先沉淀与含apo B脂蛋白相关的apo E，同时使高密度脂蛋白（HDL）中含apo E的部分留在溶液中。在对三名受试者进行的更详细分析中，我们通过琼脂糖珠柱色谱法测定了血浆沉淀前后apo E与脂蛋白的结合情况。这项研究证实了与HDL脂蛋白相关的apo E具有优先溶解性。我们使用两名血脂正常受试者的血浆，将肝素浓度维持在1.30 g/L，并将锰离子浓度从9.2 mmol/L变化至184 mmol/L。46 mmol/L的浓度能最佳地分离含apo E的HDL与含apo B的脂蛋白。因此，在这些最终浓度下，肝素 - 锰离子似乎沉淀了与含apo B脂蛋白相关的apo E，使与HDL大小脂蛋白相关的大部分apo E保持可溶状态。
