Assmann G, Schriewer H, Funke H
J Clin Chem Clin Biochem. 1981 May;19(5):273-8.
With optimal concentrations of reagents a complete precipitation of apolipoprotein B-containing lipoproteins and only a small coprecipitation of HDL can be achieved using the phosphotungstate/MgCl2 precipitation method. The accuracy of the precipitation is not influenced by triglyceride concentrations less than 4.0 mmol/l. Similarly, in most hypertriglyceridaemic sera (greater than 4.0 mmol/l) a complete precipitation of apolipoprotein B-containing lipoproteins and a complete recovery of HDL can be observed. In addition to the HDL cholesterol determination, apolipoprotein A-I was determined by kinetic nephelometry. In hypertriglyceridaemic sera (greater than 4.56 mmol/l) precipitation of apolipoprotein B-containing lipoproteins must be carried out prior to the nephelometric determination of apolipoprotein A-I.
使用磷钨酸盐/MgCl2沉淀法，在试剂浓度最佳的情况下，可实现含载脂蛋白B的脂蛋白完全沉淀，而高密度脂蛋白（HDL）仅有少量共沉淀。当甘油三酯浓度低于4.0 mmol/l时，沉淀的准确性不受影响。同样，在大多数高甘油三酯血症血清（大于4.0 mmol/l）中，可观察到含载脂蛋白B的脂蛋白完全沉淀以及HDL的完全回收。除了测定HDL胆固醇外，载脂蛋白A-I通过动力学散射比浊法测定。在高甘油三酯血症血清（大于4.56 mmol/l）中，在散射比浊法测定载脂蛋白A-I之前，必须先进行含载脂蛋白B的脂蛋白沉淀。
