gamma-Hexachlorocyclohexane activation of alveolar macrophage phosphatidylinositol cycle, calcium mobilization of O2- production.

The influence of gamma-hexachlorocyclohexane (HCC) on phosphatidyl inositol (PI) cycle activity was investigated in the guinea pig alveolar macrophage. Similar to stimulation by the chemotactic peptide N-formyl-nle-leu-phe (FNLLP), 125 microM HCC was found to stimulate PI cycle activity, calcium mobilization (45Ca2+ efflux and cytosolic [Ca2+] elevation) and O-2 production, although the action of HCC was prolonged in comparison. HCC treatment did not block subsequent stimulation by FNLLP. HCC is proposed to act at an early stage in the same activation sequence as does FNLLP.