Holmes S D, Lipshultz L I, Smith R G
J Clin Endocrinol Metab. 1984 Dec;59(6):1058-62. doi: 10.1210/jcem-59-6-1058.
Transferrin represents 1-4% of the total proteins secreted by human Sertoli cells, and the amount secreted by the cells was maximal after a 4- to 5-day period in culture, the time chosen for each medium change. During this first 4- to 5-day period, the addition of FSH, insulin, (Bu)2cAMP, and isobutylmethylxanthine had no effect on transferrin secretion; however, from days 4-5 to 8-10, each of the above compounds significantly stimulated transferrin secretion compared to control values. Testosterone (in the absence or presence of insulin) had no effect. Transferrin secretion increased for the first 5 days in culture, with a similar magnitude in the presence or absence of the above stimulators, and thereafter declined, more so in untreated cultures. These results suggest that these agents do not stimulate, but, rather, limit the decline in transferrin secretion. When human peritubular cells were cocultured with Sertoli cells, transferrin secretion was significantly elevated compared to that by Sertoli cells alone. Interestingly, in the cocultures (Bu)2cAMP stimulated transferrin secretion when added for the first 4- to 5-day culture period. Human fibroblasts or spent medium from the peritubular cell cultures did not mimic the effect found when peritubular and Sertoli cells were cocultured. These results provide evidence that peritubular cells play a critical role in regulating human Sertoli cell function.
转铁蛋白占人类支持细胞分泌的总蛋白的1%-4%，细胞分泌量在培养4至5天后达到最大，这也是每次更换培养基的时间。在最初的4至5天培养期内，添加促卵泡激素、胰岛素、二丁酰环磷腺苷（Bu）2cAMP和异丁基甲基黄嘌呤对转铁蛋白分泌没有影响；然而，从第4至5天到第8至10天，与对照值相比，上述每种化合物都显著刺激了转铁蛋白的分泌。睾酮（无论有无胰岛素）没有影响。在培养的前5天，转铁蛋白分泌增加，无论有无上述刺激物，增加幅度相似，此后下降，在未处理的培养物中下降得更多。这些结果表明，这些物质不是刺激转铁蛋白分泌，而是限制其分泌的下降。当人类睾丸间质细胞与支持细胞共培养时，转铁蛋白分泌比单独培养的支持细胞显著升高。有趣的是，在共培养中，在最初4至5天的培养期添加（Bu）2cAMP会刺激转铁蛋白分泌。人类成纤维细胞或睾丸间质细胞培养的用过的培养基并不能模拟睾丸间质细胞与支持细胞共培养时发现的效果。这些结果证明睾丸间质细胞在调节人类支持细胞功能中起关键作用。
