Hutson J C, Stocco D M
Endocrinology. 1981 Apr;108(4):1362-8. doi: 10.1210/endo-108-4-1362.
The efficiency of androgen-binding protein (ABP) secretion into the medium of cultured Sertoli cells was increased by the addition of peritubular cells into the culture system. There was a positive correlation between the number of peritubular cells added to the cultures and the amount of ABP secreted. These responses were observed in cultures treated with highly purified FSH or in untreated cultures. Medium that had been exposed to peritubular cells before exposure to Sertoli cells did not mimic the effect observed when peritubular cells were added directly to Sertoli cells. The efficiency of ABP secretion by Sertoli cells was not a function of plating density, since the amount of ABP per mg cellular protein did not vary significantly among cultures of Sertoli cells which contained a wide variation in the numbers of cells present. These results suggest that peritubular cells play a role in regulating ABP secretion and thus support the hypothesis that cell to cell communication exists between these two cell types of the seminiferous tubule.
将睾丸间质细胞添加到培养系统中，可提高培养的支持细胞向培养基中分泌雄激素结合蛋白（ABP）的效率。添加到培养物中的睾丸间质细胞数量与分泌的ABP量之间存在正相关。在用高度纯化的促卵泡激素（FSH）处理的培养物或未处理的培养物中均观察到了这些反应。在暴露于支持细胞之前先暴露于睾丸间质细胞的培养基，无法模拟将睾丸间质细胞直接添加到支持细胞时所观察到的效果。支持细胞分泌ABP的效率不是接种密度的函数，因为在细胞数量差异很大的支持细胞培养物中，每毫克细胞蛋白的ABP量并无显著差异。这些结果表明，睾丸间质细胞在调节ABP分泌中发挥作用，从而支持了生精小管的这两种细胞类型之间存在细胞间通讯的假说。
