以脂多糖为抗原，采用酶联免疫吸附测定法对兔源多杀性巴氏杆菌五种血清型进行血清学分析。
Serological analysis of five serotypes of Pasteurella multocida of rabbit origin by use of an enzyme-linked immunosorbent assay with lipopolysaccharide as antigen.
作者信息
Cary C J, Peter G K, Chrisp C E, Keren D F
出版信息
J Clin Microbiol. 1984 Aug;20(2):191-4. doi: 10.1128/jcm.20.2.191-194.1984.
Abstract
The serological relationships among five Pasteurella multocida strains, representing five somatic serotypes most commonly isolated from rabbits (serotypes 1, 3, 4, 12, and 15), were studied with an enzyme-linked immunosorbent assay. Lipopolysaccharides from the five serotypes were used as antigens in the assay. Antisera against serotypes 1 and 15 reacted only with their homologous lipopolysaccharides. Significant cross-reactivity was found between serotypes 4 and 12. Serotype 3 antisera showed minimal reactivity with both homologous and heterologous lipopolysaccharides. The feasibility of detecting antibodies to each of several lipopolysaccharide antigens combined in the same assay cell well was demonstrated.
摘要
利用酶联免疫吸附测定法研究了代表最常从兔中分离出的五种菌体血清型（血清型1、3、4、12和15）的5株多杀巴斯德菌菌株之间的血清学关系。在该测定中，使用来自这五种血清型的脂多糖作为抗原。抗血清型1和15的抗血清仅与其同源脂多糖发生反应。血清型4和12之间发现了显著的交叉反应性。血清型3抗血清对同源和异源脂多糖均显示出最小的反应性。证明了在同一测定细胞孔中联合检测针对几种脂多糖抗原中每一种的抗体的可行性。
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