Ward K A, Everson J S, Lambden P R, Watt P J
J Gen Virol. 1984 Oct;65 ( Pt 10):1749-57. doi: 10.1099/0022-1317-65-10-1749.
Antigenic and structural variation in the major nucleocapsid protein, VPN41, from different strains of respiratory syncytial (RS) virus was observed using a combination of monoclonal antibodies and two-dimensional peptide mapping. Limited trypsin treatment of intact nucleocapsids produced two peptide fragments Mr 27K and Mr 14K. Two monoclonal antibodies, N1 and N2, reactive with primary sequence epitopes located on intact nucleocapsids also reacted with either the 27K fragment (N2) or the 14K fragment (N1). Competitive radioimmunoassay studies using N1 and N2 antibodies revealed two discrete antigenic groups among the seven human strains of RS virus examined. A bovine strain of RS virus, although antigenically similar to the human strain of RS virus, was placed in a separate group. Two-dimensional peptide mapping of 125I-labelled VPN41 purified by SDS-PAGE revealed extensive structural homology between all strains. However, several unique tryptic/chymotryptic peptides supported the grouping obtained with the monoclonal antibodies.
利用单克隆抗体和二维肽图相结合的方法，观察了来自不同呼吸道合胞（RS）病毒株的主要核衣壳蛋白VPN41的抗原性和结构变异。对完整核衣壳进行有限的胰蛋白酶处理产生了两个肽片段，分子量分别为27K和14K。两种与完整核衣壳上的一级序列表位反应的单克隆抗体N1和N2，也分别与27K片段（N2）或14K片段（N1）反应。使用N1和N2抗体进行的竞争性放射免疫分析研究表明，在所检测的7株人RS病毒中存在两个不同的抗原组。一株牛RS病毒，尽管在抗原性上与人类RS病毒株相似，但被归为一个单独的组。通过SDS-PAGE纯化的125I标记的VPN41的二维肽图显示，所有毒株之间存在广泛的结构同源性。然而，一些独特的胰蛋白酶/胰凝乳蛋白酶肽支持了用单克隆抗体获得的分组。
