Ward K A, Lambden P R, Ogilvie M M, Watt P J
J Gen Virol. 1983 Sep;64 (Pt 9):1867-76. doi: 10.1099/0022-1317-64-9-1867.
The human antibody response to respiratory syncytial (RS) virus infection was investigated using radioimmunoprecipitation analysis (RIPA). A total of nine RS virus-specific proteins, VP200, VGP95, VP68, VGP48, VPN41, VP35, VP27, VP23 and VGP20 were identified by comparing 35S- or 3H-labelled extracts of infected and uninfected HEp-2 cells, and by radioimmunoprecipitation using a hyperimmune human serum. Three glycopeptides, VGP95, VGP48 and VGP20, were identified by incorporation of [3H]glucosamine, and two of these (VGP48 and VGP20) were assumed to be part of a single disulphide-bonded polypeptide since they were precipitated by a monoclonal antibody raised against a surface protein. Human serum antibodies to three major RS virus proteins, VGP95, VGP48/VGP20 and VPN41 were measured by RIPA using radioiodinated RS virus antigens. Sera from a group of mothers whose babies escaped RS virus infection during a local epidemic showed increased antibody levels to VPN41 when compared to sera from mothers whose babies had become infected with RS virus within the first 6 months of life. In infants who remained uninfected with RS virus during the first 12 months of life the maternal gift of antibody decayed to about 50% at 3 months with traces of antibodies detected in a few sera at 12 months. The antibody levels detected in the sera of infants less than 3 months old convalescent from primary RS virus infection did not exceed the mean levels present in the serum of uninfected babies. Infants between the ages of 6 and 12 months were able to mount an IgG response to VPN41 and VGP48 but, unlike adults and older children, a particularly striking finding was their failure to produce antibodies to VGP95.
利用放射免疫沉淀分析（RIPA）研究了人类对呼吸道合胞（RS）病毒感染的抗体反应。通过比较感染和未感染的HEp-2细胞的35S或3H标记提取物，并使用超免疫人血清进行放射免疫沉淀，共鉴定出9种RS病毒特异性蛋白，即VP200、VGP95、VP68、VGP48、VPN41、VP35、VP27、VP23和VGP20。通过掺入[3H]葡糖胺鉴定出3种糖肽，即VGP95、VGP48和VGP20，其中两种（VGP48和VGP20）被认为是单一二硫键连接多肽的一部分，因为它们被针对表面蛋白产生的单克隆抗体沉淀。使用放射性碘化的RS病毒抗原，通过RIPA测定了人类血清中针对3种主要RS病毒蛋白VGP95、VGP48/VGP20和VPN41的抗体。与在生命的前6个月内婴儿感染RS病毒的母亲的血清相比，一组在当地疫情期间婴儿未感染RS病毒的母亲的血清显示出针对VPN41的抗体水平升高。在生命的前12个月内未感染RS病毒的婴儿中，母体给予的抗体在3个月时衰减至约50%，在12个月时在少数血清中检测到微量抗体。从原发性RS病毒感染中康复的3个月以下婴儿血清中检测到的抗体水平未超过未感染婴儿血清中的平均水平。6至12个月大的婴儿能够对VPN41和VGP48产生IgG反应，但与成人和大龄儿童不同的是，一个特别显著的发现是他们未能产生针对VGP95的抗体。
