Watson S P, Iversen L L
Regul Pept. 1984 Jul;8(4):273-81. doi: 10.1016/0167-0115(84)90036-3.
Specific binding of 3H-substance P was studied in guinea-pig ileum longitudinal smooth muscle membranes. A single population of non-interacting sites with an apparent dissociation constant of 1.8 nM was observed. The relative potencies of some structural analogues of substance P, notably eledoisin and substance P (5-11), in competing for 3H-substance P binding sites, however, had little resemblance to their potencies in contracting the intact muscle or in eliciting the breakdown of inositol phospholipids in this tissue. The results are discussed in the light of other binding studies for substance P.
在豚鼠回肠纵行平滑肌膜中研究了³H-物质P的特异性结合。观察到存在一群非相互作用位点，其表观解离常数为1.8 nM。然而，物质P的一些结构类似物，特别是eledoisin和物质P(5-11)，在竞争³H-物质P结合位点时的相对效力，与它们在收缩完整肌肉或引发该组织中肌醇磷脂分解的效力几乎没有相似之处。根据物质P的其他结合研究对结果进行了讨论。
