Van de Kar L D, Lorens S A, McWilliams C R, Kunimoto K, Urban J H, Bethea C L
Brain Res. 1984 Oct 8;311(2):333-41. doi: 10.1016/0006-8993(84)90096-9.
Stress-induced changes in renin and prolactin secretion were studied using a conditioned emotional response paradigm. Three minutes after being placed in a chamber, the stressed animals received a brief electric shock (1.0 mA for 10 s through the grid floor), then were returned to their home cage. This procedure was repeated for 3 consecutive days. On the fourth day, the rats were placed in the chamber for 3 min, but instead of receiving shock, they were removed and sacrificed. Control animals were treated in the same manner, except that they never received foot shock. The sham-operated stressed rats evidenced significant elevations in plasma renin activity (270%) and prolactin level (550%). Electrolytic lesions in the dorsal raphe nucleus blocked the stress-induced increase in plasma renin activity but did not affect the stress-induced increase in prolactin secretion. Electrolytic lesions in the median raphe nucleus did not affect prolactin levels in either control or stressed animals. However, median raphe lesions led to a significant increase in plasma renin activity in non-stressed rats and potentiated the stress-induced elevation in plasma renin activity. These results suggest that neurons within the dorsal and median raphe nuclei are involved in the regulation of renin but not prolactin secretion during stress. The results also suggest that median raphe neurons play a role in basal renin secretion.
使用条件性情绪反应范式研究了应激诱导的肾素和催乳素分泌变化。将应激动物放入实验箱3分钟后，给予短暂电击（通过网格地板施加1.0毫安电流，持续10秒），然后放回其饲养笼。此过程连续重复3天。在第4天，将大鼠放入实验箱3分钟，但不给予电击，而是取出并处死。对照动物以相同方式处理，只是它们从未接受过足部电击。假手术应激大鼠的血浆肾素活性（升高270%）和催乳素水平（升高550%）显著升高。中缝背核的电解损伤阻断了应激诱导的血浆肾素活性增加，但不影响应激诱导的催乳素分泌增加。中缝正中核的电解损伤对对照动物或应激动物的催乳素水平均无影响。然而，中缝正中核损伤导致非应激大鼠血浆肾素活性显著增加，并增强了应激诱导的血浆肾素活性升高。这些结果表明，在应激期间，中缝背核和中缝正中核内的神经元参与肾素分泌的调节，但不参与催乳素分泌的调节。结果还表明，中缝正中核神经元在基础肾素分泌中起作用。
