海拉CCL2细胞和PHA刺激的人淋巴细胞中期和间期核仁活性的比较。

Comparison of metaphase and interphase nucleolar activity in Hela-CCL2 cells and PHA-stimulated human lymphocytes.

作者信息

Van der Elst J, Deleener A, Verschaeve L, Kirsch-Volders M, Susanne C

出版信息

Cancer Genet Cytogenet. 1984 Nov;13(3):209-23. doi: 10.1016/0165-4608(84)90044-x.

PMID:6208996

Abstract

Metaphase and interphase nucleolar activity in cultured Hela-CCL2 cells and PHA-stimulated human lymphocytes have been studied with silver nitrate staining. In metaphase, we examined the relationship between the actual number of active NORs (AgNORs) and total number of NOR-bearing acrocentrics. Interphase silver staining over the nucleus was analyzed cytodensitometrically and morphologically. From all investigations, Hela-CCL2 cells and lymphocytes were shown to have similar levels of nucleolar activity. Our results suggest that there is a form of regulation of nucleolar activity in malignant Hela-CCL2 cells as compared to PHA-stimulated human lymphocytes.

摘要

利用硝酸银染色法对培养的Hela - CCL2细胞和PHA刺激的人淋巴细胞中的中期和间期核仁活性进行了研究。在中期，我们检测了活性核仁组织区（AgNORs）的实际数量与携带核仁组织区的近端着丝粒总数之间的关系。对细胞核上的间期银染进行了细胞密度测定和形态学分析。所有研究结果表明，Hela - CCL2细胞和淋巴细胞具有相似水平的核仁活性。我们的结果表明，与PHA刺激的人淋巴细胞相比，恶性Hela - CCL2细胞中存在一种核仁活性调节形式。