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对用银浸染的核仁蛋白有丝分裂周期的透射电子显微镜研究。

Transmission electron microscopic studies on the mitotic cycle of nucleolar proteins impregnated with silver.

作者信息

Paweletz N, Risueño M C

出版信息

Chromosoma. 1982;85(2):261-73. doi: 10.1007/BF00294970.

DOI:10.1007/BF00294970
PMID:6180865
Abstract

Hela cells were impregnated with silver according to Paweletz et al. (1967). In cells in mitosis not only the nucleolar organizer regions (NORs) are strongly impregnated but also part of the nucleolar material, which accumulates in and around the chromosomes. The treatment with adenosine, which in interphase cells spreads the nucleolar material within the nucleus, also distributes the argentophilic material in and around the chromosomes. During the reconstruction phase this material reassembles around the NORs to form parts of the new nucleolus. The silver impregnation technique clearly demonstrates that two main components are responsible for the argentophily of the nucleolus. This is in agreement with the results obtained by Lischwe et al. (1979).

摘要

根据帕韦莱茨等人(1967年)的方法,用银浸染海拉细胞。在有丝分裂的细胞中,不仅核仁组织区(NORs)被强烈浸染,而且部分核仁物质也被浸染,这些物质聚集在染色体内部和周围。用腺苷处理,在间期细胞中它会使核仁物质在细胞核内扩散,同时也会使嗜银物质分布在染色体内部和周围。在重建阶段,这种物质会在NORs周围重新组装形成新核仁的部分。银浸染技术清楚地表明,有两个主要成分导致了核仁的嗜银性。这与利施韦等人(1979年)获得的结果一致。

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The nucleolus.核仁。

本文引用的文献

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Enzymatic degradation of ribonucleoproteins of chromosomes, nucleoli and cytoplasm.染色体、核仁及细胞质核糖核蛋白的酶促降解
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用氚标记的胞苷对培养的人癌细胞(海拉S3细胞系)有丝分裂期间的核RNA进行的观察。
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Ribose nucleic acid and the metaphase chromosome.核糖核酸与中期染色体。
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Demonstration of a non-RNA nucleolar fraction by silver staining.通过银染法显示非RNA核仁组分。
Exp Cell Res. 1962 Mar;26:428-31. doi: 10.1016/0014-4827(62)90195-7.
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Further observations on the ribonucleoproteins of mitotically dividing mammalian cells.关于有丝分裂的哺乳动物细胞核糖核蛋白的进一步观察
Exp Cell Res. 1961 Jan;22:193-207. doi: 10.1016/0014-4827(61)90098-2.
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Distribution of ribonucleic acid in tumor cells during mitosis.
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Localization of phosphoprotein C23 to nucleolar structures and to the nucleolus organizer regions.磷蛋白C23在核仁结构和核仁组织区的定位。
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Electron microscopic studies of silver-strained proteins in nucleolar organizer regions: location in nucleoli of rat sympathetic neurons during light and dark periods.核仁组织区中银染蛋白的电子显微镜研究:大鼠交感神经元在光照和黑暗时期核仁中的定位。
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Cell cycle analysis and drug inhibition studies of silver staining in synchronous HeLa cells.
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