Harrison H H
Clin Chem. 1984 Dec;30(12 Pt 1):1981-4.
Kodak X-Ray Duplication film provides a relatively simple and inexpensive medium for producing full-size positive-image transparencies ("XRD images") from silver-stained two-dimensional polyacrylamide gel electrophoretograms. In these XRD images, the spot or band pattern of a polychromatic silver-stained gel is represented as black against a clear background, regardless of the color of the spot in the gel itself. Thus, XRD images may be overlapped with transillumination for visual gel-to-gel comparisons, may be easily subjected to scanning densitometry, and may have the exposure adjusted to provide the proper background for black-and-white photography. I describe here the details of the XRD method and the design of a new apparatus for producing XRD images in a wet-gel "contact-print" format, which improves the method.
柯达X射线复制胶片提供了一种相对简单且成本低廉的介质，用于从银染二维聚丙烯酰胺凝胶电泳图谱制作全尺寸正像透明片（“XRD图像”）。在这些XRD图像中，多色银染凝胶的斑点或条带模式在透明背景下呈现为黑色，而不论凝胶中斑点本身的颜色如何。因此，XRD图像可通过透照重叠以进行凝胶间的视觉比较，易于进行扫描密度测定，并且可以调整曝光以提供适合黑白摄影的背景。在此，我描述XRD方法的细节以及一种用于以湿凝胶“接触印片”格式制作XRD图像的新装置的设计，该设计改进了该方法。
