Jans D A, Hatch L, Fimmel A L, Gibson F, Cox G B
J Bacteriol. 1984 Nov;160(2):764-70. doi: 10.1128/jb.160.2.764-770.1984.
The uncF469 allele differed from normal in that a G----A base change occurred at nucleotide 77 of the uncF gene, resulting in a TAG stop codon rather than the tryptophan codon TGG. Two partial revertant strains were isolated which retained the uncF469 allele but formed a partially functional b-subunit, due to suppression of the uncF469 nonsense mutation. From the altered isoelectric points of the b-subunits from these strains, it was concluded that the suppressor gene of partial revertant strain AN1956 inserts an acidic amino acid for the TAG codon, and that the suppressor gene of partial revertant strain AN1958 inserts a basic amino acid. The membranes of both partial revertant strains showed impaired permeability to protons on removal of F1-ATPase. The membranes of both strains, however, were able to carry out oxidative phosphorylation, and the ATPase activities of both were resistant to the inhibitor dicyclohexylcarbodiimide.
uncF469等位基因与正常情况不同，在于uncF基因的第77个核苷酸处发生了G----A碱基变化，导致产生TAG终止密码子而非色氨酸密码子TGG。分离出了两个部分回复突变株，它们保留了uncF469等位基因，但由于uncF469无义突变的抑制作用而形成了部分功能的β亚基。从这些菌株的β亚基改变的等电点可以得出结论，部分回复突变株AN1956的抑制基因在TAG密码子处插入了一个酸性氨基酸，而部分回复突变株AN1958的抑制基因插入了一个碱性氨基酸。去除F1 - ATP酶后，两个部分回复突变株的膜对质子的通透性受损。然而，两个菌株的膜都能够进行氧化磷酸化，并且两者的ATP酶活性都对抑制剂二环己基碳二亚胺有抗性。
