质子通透性受损的突变型大肠杆菌膜的氧化磷酸化作用
Oxidative phosphorylation by mutant Escherichia coli membranes with impaired proton permeability.
作者信息
Cox G B, Jans D A, Gibson F, Langman L, Senior A E, Fimmel A L
出版信息
Biochem J. 1983 Oct 15;216(1):143-50. doi: 10.1042/bj2160143.
Abstract
The effect on the function of the Escherichia coli F1F0-ATPase of the substitution of leucine-31 by phenylalanine in the c-subunit of the enzyme was examined. The assembly of the mutant c-subunit requires an increased gene dosage [Jans, Fimmel, Langman, James, Downie, Senior, Ash, Gibson & Cox (1983) Biochem. J. 211, 717-726], and this was achieved by incorporation of the uncE408 or uncE463 alleles on to F-plasmids or multicopy plasmids. Membranes from strains carrying either the uncE463 or uncE408 alleles on F-plasmids or multicopy plasmids were capable of carrying out oxidative phosphorylation. In particular, membranes from strain AN1928 (pAN162, uncE463) gave phosphorylation rates and P/O ratios equal to or greater than those obtained for the control strain AN1460 (pAN45, unc+). However, the mutant membranes, on removal of the F1-ATPase, appeared to be proton-impermeable. The ATPase activity of the mutant membranes was also resistant to the inhibitor dicyclohexylcarbodi-imide.
摘要
研究了将该酶c亚基中的亮氨酸-31替换为苯丙氨酸对大肠杆菌F1F0 - ATP酶功能的影响。突变型c亚基的组装需要增加基因剂量[扬斯、菲默尔、朗曼、詹姆斯、唐尼、西尼尔、阿什、吉布森和考克斯（1983年）《生物化学杂志》211卷，717 - 726页]，这是通过将uncE408或uncE463等位基因整合到F质粒或多拷贝质粒上来实现的。携带F质粒或多拷贝质粒上uncE463或uncE408等位基因的菌株的膜能够进行氧化磷酸化。特别地，菌株AN1928（pAN162，uncE463）的膜给出的磷酸化速率和P/O比等于或大于对照菌株AN1460（pAN45，unc +）所获得的速率和比值。然而，去除F1 - ATP酶后，突变型膜似乎对质子不可渗透。突变型膜的ATP酶活性也对抑制剂二环己基碳二亚胺具有抗性。
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