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1
Oxidative phosphorylation by mutant Escherichia coli membranes with impaired proton permeability.质子通透性受损的突变型大肠杆菌膜的氧化磷酸化作用
Biochem J. 1983 Oct 15;216(1):143-50. doi: 10.1042/bj2160143.
2
An additional acidic residue in the membrane portion of the b-subunit of the energy-transducing adenosine triphosphatase of Escherichia coli affects both assembly and function.大肠杆菌能量转换型三磷酸腺苷酶β亚基膜部分的一个额外酸性残基会影响组装和功能。
Biochem J. 1984 Jul 1;221(1):43-51. doi: 10.1042/bj2210043.
3
Mutations in the uncE gene affecting assembly of the c-subunit of the adenosine triphosphatase of Escherichia coli.影响大肠杆菌三磷酸腺苷酶c亚基装配的uncE基因突变。
Biochem J. 1983 Jun 1;211(3):717-26. doi: 10.1042/bj2110717.
4
Integration of F1 and the membrane sector of the proton-ATPase of Escherichia coli. Role of subunit "b" (uncF protein).F1 与大肠杆菌质子 -ATP 酶膜部分的整合。亚基“b”（uncF 蛋白）的作用。
J Biol Chem. 1983 Aug 25;258(16):9793-800.
5
The F1F0-ATPase of Escherichia coli. Substitution of proline by leucine at position 64 in the c-subunit causes loss of oxidative phosphorylation.大肠杆菌的F1F0 - ATP合酶。c亚基第64位的脯氨酸被亮氨酸取代会导致氧化磷酸化作用丧失。
Biochem J. 1983 Aug 1;213(2):451-8. doi: 10.1042/bj2130451.
6
A fifth gene (uncE) in the operon concerned with oxidative phosphorylation in Escherichia coli.大肠杆菌中与氧化磷酸化相关的操纵子中的第五个基因（uncE）。
J Bacteriol. 1979 Feb;137(2):711-8. doi: 10.1128/jb.137.2.711-718.1979.
7
An acidic or basic amino acid at position 26 of the b subunit of Escherichia coli F1F0-ATPase impairs membrane proton permeability: suppression of the uncF469 nonsense mutation.大肠杆菌F1F0 - ATP酶β亚基第26位的酸性或碱性氨基酸会损害膜质子通透性：uncF469无义突变的抑制作用。
J Bacteriol. 1984 Nov;160(2):764-70. doi: 10.1128/jb.160.2.764-770.1984.
8
The F1F0-ATPase of Escherichia coli. The substitution of alanine by threonine at position 25 in the c-subunit affects function but not assembly.大肠杆菌的F1F0 - ATP合酶。c亚基第25位的丙氨酸被苏氨酸取代会影响功能，但不影响组装。
Biochim Biophys Acta. 1985 Jul 17;808(2):252-8. doi: 10.1016/0005-2728(85)90007-6.
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The F1F0-ATPase of Escherichia coli. The substitution of alanine by tyrosine at position 25 in the c-subunit affects function but not assembly.大肠杆菌的F1F0 - ATP合酶。c亚基第25位的丙氨酸被酪氨酸取代会影响功能，但不影响组装。
Biochim Biophys Acta. 1989 Jan 30;978(2):299-304. doi: 10.1016/0005-2736(89)90128-4.
10
Mutations within the uncE gene affecting assembly of the F1F0-ATPase of Escherichia coli.影响大肠杆菌F1F0 - ATP酶组装的uncE基因内的突变。
Biochem J. 1990 Jul 15;269(2):303-8. doi: 10.1042/bj2690303.

引用本文的文献

1
H+/ATP stoichiometry of cowpea Rhizobium sp. strain 32H1 cells grown under nitrogen-fixing and nitrogen-nonfixing conditions.豇豆根瘤菌32H1菌株在固氮和非固氮条件下生长的细胞中H⁺/ATP化学计量比
J Bacteriol. 1984 Oct;160(1):216-21. doi: 10.1128/jb.160.1.216-221.1984.
2
An additional acidic residue in the membrane portion of the b-subunit of the energy-transducing adenosine triphosphatase of Escherichia coli affects both assembly and function.大肠杆菌能量转换型三磷酸腺苷酶β亚基膜部分的一个额外酸性残基会影响组装和功能。
Biochem J. 1984 Jul 1;221(1):43-51. doi: 10.1042/bj2210043.
3
An acidic or basic amino acid at position 26 of the b subunit of Escherichia coli F1F0-ATPase impairs membrane proton permeability: suppression of the uncF469 nonsense mutation.大肠杆菌F1F0 - ATP酶β亚基第26位的酸性或碱性氨基酸会损害膜质子通透性：uncF469无义突变的抑制作用。
J Bacteriol. 1984 Nov;160(2):764-70. doi: 10.1128/jb.160.2.764-770.1984.
4
Altered translation of the uncC gene coding for the epsilon subunit of the F1F0-ATPase of Escherichia coli.编码大肠杆菌F1F0 - ATP酶ε亚基的uncC基因的翻译改变。
J Bacteriol. 1987 Jul;169(7):2945-9. doi: 10.1128/jb.169.7.2945-2949.1987.
5
Complementation between uncF alleles affecting assembly of the F1F0-ATPase complex of Escherichia coli.影响大肠杆菌F1F0 - ATP酶复合体组装的uncF等位基因之间的互补作用。
J Bacteriol. 1985 Apr;162(1):420-6. doi: 10.1128/jb.162.1.420-426.1985.
6
Mutations within the uncE gene affecting assembly of the F1F0-ATPase of Escherichia coli.影响大肠杆菌F1F0 - ATP酶组装的uncE基因内的突变。
Biochem J. 1990 Jul 15;269(2):303-8. doi: 10.1042/bj2690303.
7
Second-site revertants of an arginine-210 to lysine mutation in the a subunit of the F0F1-ATPase from Escherichia coli: implications for structure.大肠杆菌F0F1 - ATP酶α亚基中精氨酸210突变为赖氨酸的二次位点回复突变体：对结构的影响
Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9799-803. doi: 10.1073/pnas.89.20.9799.
8
Mutational analysis of the glycine-rich region of the c subunit of the Escherichia coli F0F1 ATPase.大肠杆菌F0F1 ATP酶c亚基富含甘氨酸区域的突变分析
J Bacteriol. 1992 Jul;174(13):4496-9. doi: 10.1128/jb.174.13.4496-4499.1992.

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Hybridization between Escherichia coli and Shigella.大肠杆菌与志贺氏菌之间的杂交。
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The induction kinetics of bacterial photophosphorylation. Threshold effects by the phosphate potential and correlation with the amplitude of the carotenoid absorption band shift.细菌光合磷酸化的诱导动力学。磷酸盐电位的阈值效应以及与类胡萝卜素吸收带位移幅度的相关性。
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4
The defective proton-ATPase of uncA mutants of Escherichia coli. Studies of nucleotide binding sites, bound aurovertin fluorescence, and labeling of essential residues of the purified F1-ATPase.大肠杆菌uncA突变体中存在缺陷的质子-ATP酶。核苷酸结合位点的研究、结合金褐霉素的荧光以及纯化的F1-ATP酶必需残基的标记。
J Biol Chem. 1981 Oct 25;256(20):10383-9.
5
Three genes coding for subunits of the membrane sector (F0) of the Escherichia coli adenosine triphosphatase complex.编码大肠杆菌三磷酸腺苷酶复合体膜部（F0）亚基的三个基因。
J Bacteriol. 1981 Jan;145(1):200-10. doi: 10.1128/jb.145.1.200-210.1981.
6
Subunits of the adenosine triphosphatase complex translated in vitro from the Escherichia coli unc operon.从大肠杆菌unc操纵子体外翻译的三磷酸腺苷酶复合物的亚基。
J Bacteriol. 1980 Jul;143(1):8-17. doi: 10.1128/jb.143.1.8-17.1980.
7
Amino acid replacement in dicyclohexylcarbodiimide-reactive proteins from mutant strains of Escherichia coli defective in the energy-transducing ATPase complex.来自能量转换型ATP酶复合体存在缺陷的大肠杆菌突变菌株的二环己基碳二亚胺反应性蛋白质中的氨基酸置换。
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8
The proteolipid of a mutant ATPase from Escherichia coli defective in H+-conduction contains a glycine instead of the carbodiimide-reactive aspartyl residue.来自大肠杆菌的一种在质子传导方面存在缺陷的突变型ATP酶的蛋白脂质含有一个甘氨酸，而非碳二亚胺反应性天冬氨酰残基。
FEBS Lett. 1980 Jan 1;109(1):107-11. doi: 10.1016/0014-5793(80)81321-4.
9
Mutations in the uncE gene affecting assembly of the c-subunit of the adenosine triphosphatase of Escherichia coli.影响大肠杆菌三磷酸腺苷酶c亚基装配的uncE基因突变。
Biochem J. 1983 Jun 1;211(3):717-26. doi: 10.1042/bj2110717.
10
Gene order and gene-polypeptide relationships of the proton-translocating ATPase operon (unc) of Escherichia coli.大肠杆菌质子转运ATP酶操纵子（unc）的基因顺序及基因与多肽的关系
Proc Natl Acad Sci U S A. 1982 Jan;79(2):320-4. doi: 10.1073/pnas.79.2.320.

质子通透性受损的突变型大肠杆菌膜的氧化磷酸化作用

Oxidative phosphorylation by mutant Escherichia coli membranes with impaired proton permeability.

作者信息

Cox G B, Jans D A, Gibson F, Langman L, Senior A E, Fimmel A L

出版信息

Biochem J. 1983 Oct 15;216(1):143-50. doi: 10.1042/bj2160143.

DOI:10.1042/bj2160143
PMID:6316934
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1152481/
Abstract

The effect on the function of the Escherichia coli F1F0-ATPase of the substitution of leucine-31 by phenylalanine in the c-subunit of the enzyme was examined. The assembly of the mutant c-subunit requires an increased gene dosage [Jans, Fimmel, Langman, James, Downie, Senior, Ash, Gibson & Cox (1983) Biochem. J. 211, 717-726], and this was achieved by incorporation of the uncE408 or uncE463 alleles on to F-plasmids or multicopy plasmids. Membranes from strains carrying either the uncE463 or uncE408 alleles on F-plasmids or multicopy plasmids were capable of carrying out oxidative phosphorylation. In particular, membranes from strain AN1928 (pAN162, uncE463) gave phosphorylation rates and P/O ratios equal to or greater than those obtained for the control strain AN1460 (pAN45, unc+). However, the mutant membranes, on removal of the F1-ATPase, appeared to be proton-impermeable. The ATPase activity of the mutant membranes was also resistant to the inhibitor dicyclohexylcarbodi-imide.

摘要

研究了将该酶c亚基中的亮氨酸-31替换为苯丙氨酸对大肠杆菌F1F0 - ATP酶功能的影响。突变型c亚基的组装需要增加基因剂量[扬斯、菲默尔、朗曼、詹姆斯、唐尼、西尼尔、阿什、吉布森和考克斯（1983年）《生物化学杂志》211卷，717 - 726页]，这是通过将uncE408或uncE463等位基因整合到F质粒或多拷贝质粒上来实现的。携带F质粒或多拷贝质粒上uncE463或uncE408等位基因的菌株的膜能够进行氧化磷酸化。特别地，菌株AN1928（pAN162，uncE463）的膜给出的磷酸化速率和P/O比等于或大于对照菌株AN1460（pAN45，unc +）所获得的速率和比值。然而，去除F1 - ATP酶后，突变型膜似乎对质子不可渗透。突变型膜的ATP酶活性也对抑制剂二环己基碳二亚胺具有抗性。