Geoffroy J S, Becker R P
J Submicrosc Cytol. 1984 Oct;16(4):625-9.
A method of perfusion-fixation of bone marrow using a commercially available blood substitute as a vehicle for glutaraldehyde has been developed. Low magnification observations of sections by transmission electron microscopy revealed a high degree of integrity throughout the hematopoietic tissue. The continuity and fine structure of the sinusoidal endothelial cells were well preserved as evidenced by exclusion of ruthenium red/OsO4, and occasionally, stages of blood cell egress (diapedesis) across the sinusoidal lining were observed. The quality of fixation of cellular organelles was judged to be equal or superior to that obtained with the use of conventional buffer-based fixatives.
已开发出一种使用市售血液替代品作为戊二醛载体对骨髓进行灌注固定的方法。通过透射电子显微镜对切片进行低倍观察发现，造血组织整体具有高度完整性。钌红/四氧化锇排斥实验证明，窦状内皮细胞的连续性和精细结构保存良好，偶尔还观察到血细胞穿过窦状内衬的逸出（血细胞渗出）阶段。细胞器的固定质量被判定与使用传统缓冲液固定剂所获得的质量相当或更优。
