Story M T, Jacobs S C, Lawson R K
J Urol. 1984 Dec;132(6):1212-5. doi: 10.1016/s0022-5347(17)50107-x.
A 5-step fractionation procedure was used to isolate a prostatic growth factor from a tissue homogenate of human benign prostatic hyperplasia. The procedure made use of the property of prostatic growth factor to occur predominately as a large molecule (greater than 67,000) in low ionic strength solution (Tris with 0.05 M NaCl) but as a smaller molecule (less than 33,000) in high salt buffer (Tris with 1.55 M NaCl). The fractionation scheme consisted of ammonium sulfate precipitation, gel filtration (low ionic strength) and DEAE chromatography. The growth factor activity was eluted from DEAE with high ionic strength buffer followed by 2 gel filtration steps in high ionic strength buffer. The fractionation scheme produced greater than a 200-fold increase in prostatic growth factor activity and 10 per cent recovery. Additional separation steps will be required to purify prostatic growth factor to homogeneity.
采用五步分级分离程序从人良性前列腺增生组织匀浆中分离出一种前列腺生长因子。该程序利用了前列腺生长因子的特性，即在低离子强度溶液（含0.05 M氯化钠的Tris）中主要以大分子（大于67,000）形式存在，而在高盐缓冲液（含1.55 M氯化钠的Tris）中以小分子（小于33,000）形式存在。分级分离方案包括硫酸铵沉淀、凝胶过滤（低离子强度）和DEAE色谱法。生长因子活性用高离子强度缓冲液从DEAE上洗脱下来，随后在高离子强度缓冲液中进行两步凝胶过滤。该分级分离方案使前列腺生长因子活性提高了200倍以上，回收率为10%。需要额外的分离步骤将前列腺生长因子纯化至均一性。
