Dignass A, Holldorf A W
Institute of Physiological Chemistry, Ruhr-University Bochum, FRG.
Urol Res. 1992;20(2):133-8. doi: 10.1007/BF00296525.
A growth factor capable of stimulating DNA synthesis of Balb/c 3T3 cells was purified by heparin-Sepharose column chromatography about 1900-fold from the cytosol of human prostatic tissues obtained at autopsy or open prostatectomy. This growth factor bound to heparin-Sepharose in the presence of 0.5 mol/l NaCl and was eluted by 1.0-1.55 mol/l NaCl. Its molecular weight was estimated to be 68,000 by SDS-polyacrylamide gel electrophoresis. The amino acid composition was determined and compared with the data of other growth factors, which revealed no striking conformity. Distribution of growth factor activity was investigated in mechanically separated prostatic tissues of benign prostatic hyperplasia. The separation scheme provided two fractions: the stromal fraction consisting mainly of fibroblasts, fibers and smooth muscle, and the epithelial fraction consisting of epithelial cells. The specific growth-stimulating activity in the stromal fraction was about 2-fold that in the epithelial fraction. Referred to the total activity of whole tissue, about 74% of the activity could be detected in the stromal fraction, while only about 5% was detectable in the epithelial fraction. This study demonstrates the existence of a growth factor in human benign hyperplastic prostatic tissues, showing a remarkable distribution of growth factor activity, which may play a role in the pathogenesis of benign prostatic hyperplasia.
一种能够刺激Balb/c 3T3细胞DNA合成的生长因子，通过肝素-琼脂糖柱层析从尸检或开放性前列腺切除术中获得的人前列腺组织的胞质溶胶中纯化了约1900倍。这种生长因子在0.5mol/L NaCl存在下与肝素-琼脂糖结合，并通过1.0 - 1.55mol/L NaCl洗脱。通过SDS-聚丙烯酰胺凝胶电泳估计其分子量为68,000。测定了氨基酸组成并与其他生长因子的数据进行比较，结果显示没有明显的一致性。在机械分离的良性前列腺增生前列腺组织中研究了生长因子活性的分布。分离方案提供了两个部分：主要由成纤维细胞、纤维和平滑肌组成的基质部分，以及由上皮细胞组成的上皮部分。基质部分的特定生长刺激活性约为上皮部分的2倍。相对于整个组织的总活性，约74%的活性可在基质部分检测到，而在上皮部分仅约5%可检测到。本研究证明了人良性增生性前列腺组织中存在一种生长因子，其生长因子活性分布显著，可能在良性前列腺增生的发病机制中起作用。
