Zorin N A, Zorina R M
Vopr Med Khim. 1984 Jul-Aug;30(4):26-8.
A procedure is described for study of reactions between heparin and human blood serum proteins using zonal electrophoresis in agar gel containing various amounts of heparin. The alterations occurring were registered by means of cross immunoelectrophoresis. A shape of the pregnancy protein precipitates and their electrophoretic mobility were altered depending on concentration of the glycosaminoglycan present. Characteristic properties of reactions between heparin and pregnancy proteins are discussed as well as the perspectives of the use of this method for study of proteins reacting with heparin.
本文描述了一种利用在含有不同量肝素的琼脂凝胶中进行区带电泳来研究肝素与人体血清蛋白之间反应的方法。通过交叉免疫电泳记录所发生的变化。妊娠蛋白沉淀的形状及其电泳迁移率根据所存在的糖胺聚糖浓度而改变。文中讨论了肝素与妊娠蛋白之间反应的特性,以及该方法用于研究与肝素反应的蛋白质的前景。