Claassen E, Van Rooijen N
J Immunol Methods. 1984 Dec 14;75(1):181-8. doi: 10.1016/0022-1759(84)90237-0.
After injection of TNP-KLH in mice and TNP-BGG-PEN in rabbits, anti-TNP antibody-forming cells were observed in the spleen. When cryostat sections of the stimulated spleen were incubated with TNP-HRP conjugate, and then treated for HRP cytochemistry, the cytoplasm of anti-TNP-forming cells was stained red. When similar sections were incubated with TNP-AP conjugate, and then treated for AP cytochemistry, the cytoplasm of anti-TNP-forming cells was stained blue. After simultaneous incubation with TNP-AP conjugate and PEN-HSA-HRP conjugate, followed by treatment for HRP cytochemistry and AP cytochemistry, anti-TNP-forming cells with a blue cytoplasm and anti-PEN-forming cells with a red cytoplasm could be distinguished in the same spleen section.
给小鼠注射TNP-KLH以及给兔子注射TNP-BGG-PEN后，在脾脏中观察到了抗TNP抗体形成细胞。当将受刺激脾脏的冰冻切片与TNP-HRP偶联物孵育，然后进行HRP细胞化学处理时，抗TNP形成细胞的细胞质被染成红色。当将类似的切片与TNP-AP偶联物孵育，然后进行AP细胞化学处理时，抗TNP形成细胞的细胞质被染成蓝色。在与TNP-AP偶联物和PEN-HSA-HRP偶联物同时孵育，随后进行HRP细胞化学和AP细胞化学处理后，在同一张脾脏切片中可以区分出细胞质为蓝色的抗TNP形成细胞和细胞质为红色的抗PEN形成细胞。
