噬菌体φX174的复制机制 XIX. φX174病毒链DNA在基因组内部位点的合成起始
Mechanism of replication of bacteriophage phi X174 XIX. Initiation of phi X174 viral strand DNA synthesis at internal sites on the genome.
作者信息
Matthes M, Weisbeek P J, Denhardt D T
出版信息
J Virol. 1982 Apr;42(1):301-5. doi: 10.1128/JVI.42.1.301-305.1982.
Abstract
Bacteriophage phi X174 viral strand DNA molecules shorter than genome length found late in the infectious cycle in Escherichia coli were 5' end labeled with 32P. Hybridization of the 32P-labeled molecules to restriction enzyme fragments of phi X replicative form DNA revealed an excess of phi X molecules whose 5' ends mapped in HaeIII fragments Z3 and Z4 in comparison with fragments Z1 and Z2. This suggests that initiation of phi X174 viral strand DNA synthesis may occur at internal sites on the complementary strand. There are several appropriately located sequences that might serve as n' (factor Y) recognition sequences and thereby facilitate discontinuous synthesis of the viral strand.
摘要
在大肠杆菌感染周期后期发现的短于基因组长度的噬菌体φX174病毒链DNA分子，用32P对其5'末端进行了标记。将32P标记的分子与φX复制型DNA的限制性酶切片段进行杂交，结果显示，与片段Z1和Z2相比，5'末端定位于HaeIII片段Z3和Z4中的φX分子过量。这表明φX174病毒链DNA合成的起始可能发生在互补链的内部位点。存在几个位置合适的序列，它们可能作为n'（因子Y）识别序列，从而促进病毒链的不连续合成。
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