在质粒（ColE1和pBR322）复制起点附近鉴定出两个大肠杆菌Y因子效应位点。
Identification of two Escherichia coli factor Y effector sites near the origins of replication of the plasmids (ColE1 and pBR322.
作者信息
Zipursky S L, Marians K J
出版信息
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6521-5. doi: 10.1073/pnas.77.11.6521.
Abstract
The Escherichia coli replication factor Y has been characterized as a phi X174 (+) strand specific DNA-dependent phosphohydrolase. In conjunction with other E. coli replication proteins, factor Y is involved in the formation of heterogeneous primers that are elongated by the E. coli DNA polymerase III elongation machinery. We report here that the heat-denatured DNAs of plasmids pBR322 and ColE1 serve as effectors for the hydrolysis of ATP by factor Y. The DNA sequences of pBR322 responsible for factor Y effector activity have been localized. Two separate regions of the pBR322 chromosome support Y ATPase activity. These sequences are near the replication origin and are located on opposite DNA strands.
摘要
大肠杆菌复制因子Y已被鉴定为一种φX174（+）链特异性的依赖DNA的磷酸水解酶。与其他大肠杆菌复制蛋白一起，因子Y参与形成异源引物，这些引物由大肠杆菌DNA聚合酶III延伸机制进行延伸。我们在此报告，质粒pBR322和ColE1的热变性DNA可作为因子Y水解ATP的效应物。负责因子Y效应物活性的pBR322的DNA序列已被定位。pBR322染色体的两个独立区域支持Y ATP酶活性。这些序列靠近复制起点，且位于相反的DNA链上。
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