Borgers M, Thoné F, de Crée J, de Cock W
Histochem J. 1978 Jan;10(1):31-43. doi: 10.1007/BF01003412.
Alkaline phosphatase has been localized ultracytochemically in PMN of man with normal and elevated levels of this enzyme. Contrary to guinea-pig PMN, no activity appears to be present in the specific granules. Instead, the plasma membrane and the membrane of the endocytic vacuoles show a strong staining. However, the demonstration of this activity depends on the preparatory procedure employed for PMN isolation. The use of dextran and Ficoll-Hypaque in the isolation procedure induces a marked increase in alkaline phosphatase staining of the PMN plasma membrane. Strongly increased activity at this site has been found in PMN from cancer patients. In most of them, additional staining has been observed in atypical vesicles and sometimes in the Golgi apparatus. These findings are discussed in the light of some previously reported controversial biochemical and cytochemical data on the distribution of alkaline phosphatase in human PMN.
已经通过超微细胞化学方法将碱性磷酸酶定位在该酶水平正常和升高的人的中性粒细胞中。与豚鼠中性粒细胞相反，特异性颗粒中似乎没有活性。相反，质膜和内吞泡的膜显示出强烈的染色。然而，这种活性的显示取决于用于分离中性粒细胞的制备程序。在分离程序中使用葡聚糖和聚蔗糖-泛影葡胺会导致中性粒细胞质膜碱性磷酸酶染色显著增加。在癌症患者的中性粒细胞中发现该部位的活性大幅增加。在大多数患者中，在非典型囊泡中有时在高尔基体中观察到额外的染色。根据先前报道的关于碱性磷酸酶在人中性粒细胞中分布的一些有争议的生化和细胞化学数据对这些发现进行了讨论。
