Rausch P G, Moore T G
Blood. 1975 Dec;46(6):913-9.
The functional significance of granule enzymes in polymorphonuclear leukocytes (PMN) is not fully understood because of the multiplicity of the enzymes and the rare occurrence of deficiencies in man. In order to select appropriate laboratory animals for functional studies, a phylogenetic comparison of enzyme levels in animal and human PMN was undertaken. Neutrophils were obtained from a variety of laboratory animals and man; the activities of alkaline phosphatase, lysozyme, myeloperoxidase, and beta-glucuronidase were determined by histochemical and analytical techniques. Marked interspecies differences in enzyme activity were found; many species were deficient in alkaline phosphatase or lysozyme. Differences in pH optima and metal requirements of alkaline phosphatase were not of sufficient magnitude to explain the variations of this enzyme.
由于多形核白细胞（PMN）中颗粒酶种类繁多，且人类中酶缺乏的情况罕见，因此对其功能意义尚未完全了解。为了选择合适的实验动物进行功能研究，对动物和人类PMN中的酶水平进行了系统发育比较。从多种实验动物和人类中获取中性粒细胞；通过组织化学和分析技术测定碱性磷酸酶、溶菌酶、髓过氧化物酶和β-葡萄糖醛酸酶的活性。发现酶活性存在明显的种间差异；许多物种缺乏碱性磷酸酶或溶菌酶。碱性磷酸酶的最适pH值和金属需求差异不足以解释该酶的变化。
