Barfod N M, Schjerbeck P
Cell Tissue Kinet. 1982 Jul;15(4):457-68. doi: 10.1111/j.1365-2184.1982.tb01063.x.
The cell-specific inhibitory (chalone) activity of JB-1 ascites tumour cell proliferation has been purified using five different procedures. By combining (1) molecular weight estimations based on ultrafiltration and gel chromatography, and (2) partitioning in organic solvent and ion exchanges, it is concluded that the active factor associates, in a complex manner, with various other components involving both hydrophobic and ionic forces. The active factor appears to be slightly acidic, hydrophobic peptide (molecular weight 500-1000 D). When assessing the activity in vivo, it appears to be highly dependent on associated serum factors. Thus, the chalone studied appears to interact both structurally and functionally with various associated factors which affect its physicochemical behaviour and biological activity.
已使用五种不同方法纯化了JB-1腹水肿瘤细胞增殖的细胞特异性抑制（抑素）活性。通过结合（1）基于超滤和凝胶色谱的分子量估计，以及（2）在有机溶剂中的分配和离子交换，得出活性因子以复杂方式与涉及疏水和离子力的各种其他成分缔合的结论。活性因子似乎是略带酸性的疏水肽（分子量500-1000 D）。在体内评估活性时，它似乎高度依赖于相关的血清因子。因此，所研究的抑素似乎在结构和功能上与各种相关因子相互作用，这些因子会影响其物理化学行为和生物活性。
