Barfod N M
Cell Tissue Kinet. 1981 Jul;14(4):389-94. doi: 10.1111/j.1365-2184.1981.tb00545.x.
A flow cytometric in vivo chalone assay is described. Transplantation of old JB-1 ascites tumour cells to new hosts induced an influx of tumour cells, with G1 DNA content, to the S phase. This induction could be reversibly and specifically blocked by injections of an ultrafiltrate of old JB-1 ascites fluid. The method described is superior to a previously published in vivo chalone assay using regenerating ascites tumours. Owing to a reduced variability in time of onset of DNA synthesis, a smaller scatter of observations is achieved and thus the number of mice per group may be reduced using the new method. In contrast to the older technique, the present one does not necessitate killing of mice during the observation period.
本文描述了一种流式细胞术体内抑素测定法。将老龄JB - 1腹水肿瘤细胞移植到新宿主中会诱导DNA含量为G1期的肿瘤细胞流入S期。这种诱导作用可通过注射老龄JB - 1腹水超滤液而被可逆且特异性地阻断。所描述的方法优于先前发表的使用再生腹水肿瘤的体内抑素测定法。由于DNA合成开始时间的变异性降低，观察值的离散度更小，因此使用新方法每组小鼠的数量可以减少。与旧技术不同，目前的方法在观察期内不需要处死小鼠。
