McKee R W, Kang-Lee Y A, Panaqua M, Swendseid M E
J Chromatogr. 1982 Jul 9;230(2):309-17. doi: 10.1016/s0378-4347(00)80480-6.
A high-performance liquid chromatographic procedure has been developed for the quantitation of nicotinamide, nicotinic acid, nicotinuric acid, 1-methylnicotinamide and 1-methyl-2-pyridone-5-carboxamide in rat and human urines. The procedure utilizes a Varian Model 5020 liquid chromatograph with a UV detector, and an Altex 15 cm X 4.6 mm Ultrasphere-ODS column, employing a linear ion-pair mobile phase gradient. Solvent A contains 10 mM concentrations of pentanesulfonic acid (PSA), tetramethylammonium chloride (TMA) and KH2PO4, and solvent B contains PSA, TMA and acetonitrile. Different pH values for solvent A vary the retention times and thus the separation of the five compounds. Temperature of the system is critical. The conditions found most satisfactory were pH 3.30 and 24.5 degrees C.
已开发出一种高效液相色谱法，用于定量测定大鼠和人尿液中的烟酰胺、烟酸、烟尿酸、1-甲基烟酰胺和1-甲基-2-吡啶酮-5-甲酰胺。该方法使用配备紫外检测器的瓦里安5020型液相色谱仪和Altex 15 cm×4.6 mm超微球ODS柱，采用线性离子对流动相梯度。溶剂A含有浓度为10 mM的戊烷磺酸（PSA）、四甲基氯化铵（TMA）和KH2PO4，溶剂B含有PSA、TMA和乙腈。溶剂A的不同pH值会改变保留时间，从而实现这五种化合物的分离。系统温度至关重要。最令人满意的条件是pH 3.30和24.5℃。
