Stratford M R, Dennis M F
Gray Laboratory of the Cancer Research Campaign, Mount Vernon Hospital, Northwood, Middlesex, UK.
J Chromatogr. 1992 Nov 6;582(1-2):145-51. doi: 10.1016/0378-4347(92)80313-f.
A high-performance liquid chromatographic method is described which enables the determination of nicotinamide and eight of its possible metabolites in human and murine plasma and urine, using ion-pairing on a base-deactivated reversed-phase column. Calibration curves were linear up to 2 mumol/ml for nicotinamide and 200 nmol/ml for the metabolites; both the intra- and inter-assay relative standard deviations ranged between 1 and 8%. In murine plasma, the N-oxide was the major nicotinamide metabolite, but in man, formation of 1-methylnicotinamide and the 2- and 4-pyridones was also significant. In urine, nicotinuric acid was seen in the mouse, but no nicotinic acid metabolites were seen in man.
描述了一种高效液相色谱法，该方法能够使用碱钝化反相柱上的离子对技术，测定人和小鼠血浆及尿液中的烟酰胺及其八种可能的代谢物。烟酰胺的校准曲线在高达2 μmol/ml时呈线性，代谢物的校准曲线在高达200 nmol/ml时呈线性；批内和批间相对标准偏差均在1%至8%之间。在小鼠血浆中，N-氧化物是主要的烟酰胺代谢物，但在人体中，1-甲基烟酰胺以及2-吡啶酮和4-吡啶酮的形成也很显著。在尿液中，小鼠体内可见烟尿酸，但人体中未见烟酸代谢物。
