Brown C A
J Histochem Cytochem. 1978 Jan;26(1):22-7. doi: 10.1177/26.1.621376.
Colon tumors induced with azoxymethane in male Fischer rats were cytochemically analyzed for beta-glucuronidase using naphthol AS-B1 glucuronide (6-bromo-2-hydroxy-3-naphthoyl-O-anisidine) as a substrate and hexazonium pararosanin as a diazo reagent. This method effectively localizes the bulk of beta-glucuronidase in the surface epithelium, the lamina propria and in the endothelial cells of the lymphoid sinuses and postcapillary venules. Polypoid lesions, adenocarcinomas and mucinous adenocarcinomas show no difference in the amount or in the localization of beta-glucuronidase; however, mucinous adenocarcinomas show a slight increase in the amount of beta-glucuronidase. The few tumors that did metastasize to lymph nodes did not show any difference in their enzyme patterns. Intestinal crypts that show a change in size and shape have a definite increase in beta-glucuronidase activity. An increase in the activity of this enzyme can also be seen in well defined neoplasms as opposed to normal areas of the colon.
以1-萘酚AS-B1葡萄糖醛酸苷（6-溴-2-羟基-3-萘甲酰-O-茴香胺）为底物，以副品红六偶氮盐为重氮试剂，对用氧化偶氮甲烷诱导的雄性Fischer大鼠结肠肿瘤进行β-葡萄糖醛酸酶的细胞化学分析。该方法能有效地将大部分β-葡萄糖醛酸酶定位在表面上皮、固有层以及淋巴窦和毛细血管后微静脉的内皮细胞中。息肉样病变、腺癌和黏液腺癌在β-葡萄糖醛酸酶的含量或定位上没有差异；然而，黏液腺癌的β-葡萄糖醛酸酶含量略有增加。少数转移至淋巴结的肿瘤在酶谱上没有任何差异。大小和形状发生改变的肠隐窝，其β-葡萄糖醛酸酶活性有明显增加。与结肠正常区域相比，在明确的肿瘤中也可见到这种酶活性的增加。
