Lunney J K, Sachs D H
J Immunol. 1978 Feb;120(2):607-12.
Immunochemical analyses of radioactively labeled lymphocyte antigens from miniature swine of three different homozygous major histocompatibility (MHC) types, AA, CC, and DD, have been performed. Anti-MHC sera were incubated with lentil lectin purified Nonidet P-40 swine lymphocyte extracts. Antigen-antibody complexes were then precipitated with protein A bearing staphylococci, eluted, and electrophoresed on sodium dodecyl sulfate polyacrylamide gels. Analysis of antigens from AA or DD cells revealed peaks of 42,000, 31,000, 25,000, and 11,000 dalton m.w. Platelet absorption of the anti-MHC sera yielded antibodies that only precipitated the intermediate m.w. molecules and lysed a subpopulation of swine peripheral blood lymphocytes, suggesting that these molecules were the miniature swine analogues of murine Ia antigens. Antibodies eluted from platelets lysed all lymphocyte populations and precipitated only the 42,000 and 11,000 dalton peaks, indicating that these molecules represent the analogue of murine H-2 histocompatibility antigens, containing a heavy chain and putative swine beta2-microglobulin.
已对三种不同纯合主要组织相容性（MHC）类型AA、CC和DD的小型猪的放射性标记淋巴细胞抗原进行了免疫化学分析。将抗MHC血清与扁豆凝集素纯化的非离子去污剂P-40猪淋巴细胞提取物一起孵育。然后用带有葡萄球菌A蛋白沉淀抗原-抗体复合物，洗脱，并在十二烷基硫酸钠聚丙烯酰胺凝胶上进行电泳。对来自AA或DD细胞的抗原分析显示，分子量峰值分别为42,000、31,000、25,000和11,000道尔顿。抗MHC血清经血小板吸收后产生的抗体仅沉淀中等分子量分子，并裂解猪外周血淋巴细胞的一个亚群，这表明这些分子是小鼠Ia抗原的小型猪类似物。从血小板洗脱的抗体裂解所有淋巴细胞群体，仅沉淀42,000和11,000道尔顿的峰值，表明这些分子代表小鼠H-2组织相容性抗原的类似物，包含一条重链和假定的猪β2-微球蛋白。
