Lunney J K, Sachs D H
J Immunol. 1979 Feb;122(2):623-7.
The complexity of the I region analog associated with the MHC of miniature swine has been probed by sequential antibody precipitation studies of Ia antigens. Treatment of solubilized lymphocyte preparations from MSLA homozygotes of the DD haplotype with excess AA anti-DD alloantiserum led to precipitation of only a portion of the total Ia antigens, as determined by secondary precipitation of the remaining material with CC anti-DD serum. The presumed I region of miniature swine must therefore code for more than one Ia antigen-bearing polypeptide chain. In addition, certain mouse alloantisera that had previously been shown to react with rat Ia antigens were tested for reactivity with swine Ia antigens. Anti-Iak mouse alloantisera precipitated Ia molecules from every swine extract tested, regardless of MHC type, precluding genetic mapping studies. However, sequential precipitation studies demonstrated that the cross-reactive mouse alloantisera reacted only with a subclass of swine Ia antigens, again suggesting genetic complexity of the pig Ia locus.
通过对Ia抗原进行连续抗体沉淀研究，探究了与小型猪主要组织相容性复合体（MHC）相关的I区类似物的复杂性。用过量的AA抗DD同种异体抗血清处理DD单倍型MSLA纯合子的可溶性淋巴细胞制剂，如用CC抗DD血清对剩余物质进行二次沉淀所确定的，仅沉淀了总Ia抗原的一部分。因此，小型猪假定的I区必定编码不止一种携带Ia抗原的多肽链。此外，还测试了某些先前已证明能与大鼠Ia抗原反应的小鼠同种异体抗血清与猪Ia抗原的反应性。抗Iak小鼠同种异体抗血清能从所测试的每种猪提取物中沉淀出Ia分子，无论MHC类型如何，这使得基因定位研究无法进行。然而，连续沉淀研究表明，具有交叉反应性的小鼠同种异体抗血清仅与猪Ia抗原的一个亚类发生反应，这再次表明猪Ia基因座的遗传复杂性。
