Boutry M, Goffeau A
Eur J Biochem. 1982 Jul;125(3):471-7. doi: 10.1111/j.1432-1033.1982.tb06707.x.
Among 979 non-glycerol growers of the yeast Schizosaccharomyces pombe, 40 strains were found to be deficient in the mitochondrial ATPase activity. Three of them exhibited an alteration in either the alpha or beta subunits of the F1ATPase. The alpha subunit was not immunodetected in the A23/13 mutant. The beta subunit was not immuno-detected in the B59/1 mutant. The existence of these two mutants shows that the alpha and beta subunits can be present independently of each other in the inner mitochondrial membrane. The beta subunit of the mutant F25/28 had a slower electrophoretic mobility than that of the wild-type beta subunit. This phenotype indicates abnormal processing or specific modification of the beta subunit. All mutants showed reduced activities of the NADH-cytochrome c reductase and of the cytochrome oxidase and a decreased synthesis of cytochrome aa3 and cytochrome b. This pleiotropic phenotype appears to result from specific modifications in the mitochondrial protein synthesis. The mitochondrial synthesis of four polypeptides (three cytochrome oxidase and one cytochrome b subunits) was markedly decreased or absent while three new polypeptides (Mr = 54000, 20000 and 15000) were detected in all the mutants analysed. This observation suggests that a functional F1ATPase is necessary for the correct synthesis and/or assembly of the mitochondrially made components of the cytochrome oxidase and cytochrome b complexes.
在979株粟酒裂殖酵母非甘油生长菌株中，发现有40株线粒体ATP酶活性缺陷。其中3株F1ATP酶的α或β亚基出现改变。在A23/13突变体中未免疫检测到α亚基。在B59/1突变体中未免疫检测到β亚基。这两个突变体的存在表明α和β亚基可以在线粒体内膜中彼此独立存在。突变体F25/28的β亚基电泳迁移率比野生型β亚基慢。这种表型表明β亚基存在异常加工或特异性修饰。所有突变体的NADH-细胞色素c还原酶和细胞色素氧化酶活性均降低，细胞色素aa3和细胞色素b的合成减少。这种多效性表型似乎是由线粒体蛋白质合成中的特异性修饰导致的。四种多肽（三种细胞色素氧化酶和一种细胞色素b亚基）的线粒体合成明显减少或缺失，而在所有分析的突变体中均检测到三种新多肽（分子量分别为54000、20000和15000）。这一观察结果表明，功能性F1ATP酶对于细胞色素氧化酶和细胞色素b复合物的线粒体组成成分的正确合成和/或组装是必需的。
