Ando A, Ando I, Hiraki T, Takeshita M, Hisada K
Eur J Nucl Med. 1982;7(7):298-303. doi: 10.1007/BF00253424.
Tumor-bearing animals were injected with 111In- and 169Yb-citrate. Tumor homogenates, from which the nuclear fraction was removed, and the mitochondrial fractions of the host livers were digested with pronase P. After digestion, the supernatants of the reaction mixtures were applied to Sephadex G-100 columns. The resultant eluates were analyzed for radioactivity, protein, uronic acid, and sialic acids. Three peaks of radioactivity were obtained by gel filtration. The first peak, eluted in the void volume, contained a species whose molecular weight exceeded 40 000. The second peak consisted of substances with molecular weights of 9400-40 000. Radioactivity in the third peak was liberated 111In and 169Yb. These two nuclides in the second peak were bound to acid mucopolysaccharides and/or the sulfated carbohydrate chain of sulfated glycoprotein. It was thought that the nuclides in the first peak might be bound to some acid mucopolysaccharides. The second peak nuclides seemed to be bound to acid mucopolysaccharide that contained no uronic acids, and/or to the sulfated carbohydrate chain of sulfated glycoprotein. It was concluded that they were bound to the acid mucopolysaccharides and/or the sulfated carbohydrate chain of sulfated glycoprotein in tumor tissues and liver lysosomes.
给荷瘤动物注射111铟和169镱柠檬酸盐。去除核部分后的肿瘤匀浆以及宿主肝脏的线粒体部分用链霉蛋白酶P消化。消化后，将反应混合物的上清液应用于葡聚糖G - 100柱。对所得洗脱液进行放射性、蛋白质、糖醛酸和唾液酸分析。通过凝胶过滤获得了三个放射性峰。第一个峰在空体积中洗脱，包含一种分子量超过40000的物质。第二个峰由分子量为9400 - 40000的物质组成。第三个峰中的放射性是111铟和169镱。第二个峰中的这两种核素与酸性粘多糖和/或硫酸化糖蛋白的硫酸化碳水化合物链结合。据认为，第一个峰中的核素可能与某些酸性粘多糖结合。第二个峰中的核素似乎与不含糖醛酸的酸性粘多糖和/或硫酸化糖蛋白的硫酸化碳水化合物链结合。得出的结论是，它们与肿瘤组织和肝溶酶体中的酸性粘多糖和/或硫酸化糖蛋白的硫酸化碳水化合物链结合。
