Orosz C G, Fidelus R K, Roopenian D C, Widmer M B, Ferguson R M, Bach F H
J Immunol. 1982 Nov;129(5):1865-8.
CsA interferes in a specific manner with the expansion of T cell clones in that it inhibits the antigen-driven component of the proliferative responses made by cloned helper T cells, cloned conventional cytolytic T cells, and cloned helper-independent cytolytic T cells. Cloned helper T cells and helper-independent cytolytic T cells, which share the ability to proliferate when cultured with specific alloantigen, fail to proliferate when cultured with specific alloantigen, fail to proliferate in response to this stimulus in the presence of CsA (10 to 100 ng/ml). In contrast, the proliferation observed when these cells are cultured with exogenous growth factors (but not alloantigen) is little influenced by as much as 1000 ng/ml CsA. When cloned helper T cells or helper-independent cytotoxic T cells are cultured with alloantigen plus exogenous growth factor, additive or synergistic proliferation occurs. However, CsA (10 to 1000 ng/ml) blocks only the component of proliferation induced by alloantigen, and leaves the lymphokine-driven component intact. CsA has similar effects on the proliferation of cloned conventional cytolytic T cells. Thus, CsA separates cloned T cell proliferation into two components: one driven by contact with alloantigens, the other driven by contact with mitogenic lymphokines.
环孢素A（CsA）以一种特定的方式干扰T细胞克隆的扩增，它抑制克隆的辅助性T细胞、克隆的传统细胞毒性T细胞以及克隆的非辅助依赖性细胞毒性T细胞增殖反应中由抗原驱动的部分。克隆的辅助性T细胞和非辅助依赖性细胞毒性T细胞在与特定同种异体抗原一起培养时具有增殖能力，但在存在CsA（10至100纳克/毫升）的情况下，对这种刺激无增殖反应。相反，当这些细胞与外源性生长因子（而非同种异体抗原）一起培养时所观察到的增殖，即使在高达1000纳克/毫升的CsA存在下也几乎不受影响。当克隆的辅助性T细胞或非辅助依赖性细胞毒性T细胞与同种异体抗原加外源性生长因子一起培养时，会出现相加或协同增殖。然而，CsA（10至1000纳克/毫升）仅阻断由同种异体抗原诱导的增殖部分，而使由淋巴因子驱动的部分保持完整。CsA对克隆的传统细胞毒性T细胞的增殖有类似作用。因此，CsA将克隆的T细胞增殖分为两个部分：一部分由与同种异体抗原接触驱动，另一部分由与促有丝分裂淋巴因子接触驱动。
