实验性糖尿病和食物摄入量对大鼠肠道及血清碱性磷酸酶的影响。

Effects of experimental diabetes and food intake on rat intestine and serum alkaline phosphatase.

作者信息

Chua B, Shrago E

出版信息

J Nutr. 1978 Feb;108(2):196-202. doi: 10.1093/jn/108.2.196.

DOI:10.1093/jn/108.2.196

Abstract

Alkaline phosphatase activity of rat serum was reduced 50% by fasting the animal for 24 hours. Diabetes, induced by alloxan or streptozotocin, increased serum alkaline phosphatase 3- to 5-fold in fed rats and the elevated activity was reduced by insulin administration. In the absence of insulin, fasting alone was able to reduce the serum alkaline phosphatase of diabetic rats to control values. The elevated serum isozyme was found to be of intestinal origin by the use of appropriate inhibitors and electrophoretic mobility following neuraminidase treatment. It is concluded that food intake, particularly the hyperphagia of diabetes, plays a major role in regulating the concentration of intestine and serum alkaline phosphatase in the rat.

摘要

通过使动物禁食24小时，大鼠血清碱性磷酸酶活性降低了50%。用四氧嘧啶或链脲佐菌素诱导的糖尿病，使喂食大鼠的血清碱性磷酸酶升高了3至5倍，而给予胰岛素后升高的活性降低。在没有胰岛素的情况下，仅禁食就能将糖尿病大鼠的血清碱性磷酸酶降低到对照值。通过使用适当的抑制剂和神经氨酸酶处理后的电泳迁移率，发现升高的血清同工酶来自肠道。结论是食物摄入，尤其是糖尿病的食欲亢进，在调节大鼠肠道和血清碱性磷酸酶浓度方面起主要作用。