Schuyler M R, Steinberg D
J Lab Clin Med. 1982 Dec;100(6):932-42.
Receptors for IgG and C on PM populations can be modulated by activation. AM populations constantly exposed to environmental particulates may not be subject to changes of IgG and C receptors after activation. We compared normal and activated rabbit AMs and PMs. Activation was accomplished by treatment with intravenous BCG or intratracheal Micropolyspora faeni. We examined the ability of these AM populations to attach to (form rosettes) and phagocytize SRBC coated with varying concentration of IgG or C. Compared to normal AMs, activated AM populations formed more rosettes with SRBC coated with IgG or C and phagocytized more via the IgG receptor. PMs from BCG-treated rabbits did not form more rosettes with IgG- or C-coated SRBC but demonstrated more IgG-mediated phagocytosis than did normal PMs. C-mediated phagocytosis occurred in AM populations from only five of 25 BCG-treated rabbits and two of 20 normal rabbits. The TG broth-induced rabbit PM also exhibited a low level of C and IgG-mediated phagocytosis compared to the mouse TG broth-induced PM. We conclude that rabbit AM IgG and C receptors can be altered by events that activate AMs. C-mediated phagocytosis is much lower in rabbit macrophages (both AMs and PMs) than in activated mouse PM populations. IgG-mediated phagocytosis can be used as an index of rabbit AM activation.
肺泡巨噬细胞（AM）群体和肺巨噬细胞（PM）群体上的IgG和C受体可通过激活进行调节。持续暴露于环境颗粒的AM群体在激活后可能不会出现IgG和C受体的变化。我们比较了正常和激活状态下的兔AM和PM。通过静脉注射卡介苗（BCG）或气管内注射微小多孢菌来实现激活。我们检测了这些AM群体与包被不同浓度IgG或C的绵羊红细胞（SRBC）结合（形成玫瑰花结）和吞噬的能力。与正常AM相比，激活的AM群体与包被IgG或C的SRBC形成更多玫瑰花结，并且通过IgG受体吞噬更多。经卡介苗处理的兔的PM与包被IgG或C的SRBC并未形成更多玫瑰花结，但与正常PM相比，表现出更多的IgG介导的吞噬作用。仅25只经卡介苗处理的兔中的5只以及20只正常兔中的2只的AM群体发生了C介导的吞噬作用。与小鼠经硫乙醇酸盐肉汤（TG肉汤）诱导的PM相比，TG肉汤诱导的兔PM也表现出较低水平的C和IgG介导的吞噬作用。我们得出结论，兔AM的IgG和C受体可因激活AM的事件而改变。兔巨噬细胞（包括AM和PM）中C介导的吞噬作用比激活的小鼠PM群体中的低得多。IgG介导的吞噬作用可作为兔AM激活的指标。
