Schuyler M, Subramanyan S, Hassan M O
Department of Medicine, Case Western Reserve University, Cleveland, Ohio.
Br J Exp Pathol. 1987 Oct;68(5):743-54.
Models of hypersensitivity pneumonitis (HP) should exhibit progression of pulmonary histological abnormalities during continuing challenges. Strain II guinea-pigs were sensitized with Micropolyspora faeni and received 2, 4, or 8 weekly intratracheal (i.t.) particulate M. faeni challenges. Control animals received normal saline (NS). Four days after the last exposure, randomly selected microscopic fields of lung (200/animal) were judged to be normal or abnormal. If abnormal, the location and nature of the abnormalities were determined. Compared with NS treated guinea-pigs, those exposed to 2, 4 and 8 weekly M. faeni challenges exhibited more extensive (P less than 0.001) pulmonary histological abnormalities which involved both the intraalveolar and interstitial compartments. More extensive abnormalities in the 8 week group compared with the 4 week group were caused by increased extent of interstitial mononuclear cell infiltration. The extent of pulmonary interstitial histological abnormalities transiently (four challenges) decreases, but then increases, so that progressive pulmonary inflammation occurs during continuing challenges.
过敏性肺炎（HP）模型应在持续激发过程中表现出肺部组织学异常的进展。将II型豚鼠用嗜热放线菌致敏，并每周接受2次、4次或8次气管内（i.t.）注射嗜热放线菌颗粒的激发。对照动物接受生理盐水（NS）。最后一次暴露后4天，随机选择肺的显微镜视野（每只动物200个），判断其是否正常或异常。如果异常，则确定异常的位置和性质。与接受NS治疗的豚鼠相比，每周接受2次、4次和8次嗜热放线菌激发的豚鼠表现出更广泛（P<0.001）的肺部组织学异常，累及肺泡内和间质部分。8周组与4周组相比，更广泛的异常是由间质单核细胞浸润程度增加所致。肺部间质组织学异常的程度短暂性地（四次激发）降低，但随后增加，因此在持续激发过程中会发生进行性肺部炎症。
