Kirschbaum J
J Pharm Sci. 1978 Feb;67(2):275-6. doi: 10.1002/jps.2600670244.
A previously developed differential UV assay for 3-one-4-ene steroids involving reduction with sodium borohydride was frequently found to be less than 100% complete. Such incomplete reduction can be mimicked by additions of sodium metaborate, a product of the hydrolysis of sodium borohydride, to the reaction system. Complete reduction can be achieved by adding propylene glycol to the reduction system. The phenomenon was studied using halcinonide as a model.
先前开发的用于检测3-烯-4-甾酮的差示紫外分析法，该方法涉及用硼氢化钠进行还原，经常发现还原反应不完全，不到100%。硼氢化钠水解产物偏硼酸钠添加到反应体系中，可模拟这种不完全还原的情况。在还原体系中加入丙二醇可实现完全还原。以卤米松为模型对该现象进行了研究。
