Varma R
Biochem Exp Biol. 1978;14(1):11-5.
A reliable method for the quantitative determination of "total 17-hydroxycorticosteroids has been described. Urine is subjected to reduction with sodium borohydride which reduces the 17-hydroxy-20-keto-21-deoxy steroids to 17,20-dihydroxy-21-deoxy steroids and 17-ketosteroids to C19 17-hydroxysteroids. Subsequent oxidation with sodium bismuthate oxidizes all the C21 17-hydroxysteroids to the corresponding 17-ketosteroids, which are extracted with CHCl3 : Bu-OH using ammonium sulfate as the salting out agent and finally the color reaction is performed with m-dinitrobenzene and the absorbance is measured. The 17-ketosteroids originally present in the urine do not interfere, since NaBH4 reduces them to C19 17-hydroxysteroids which cannot be oxidized with sodium bismuthate and do not respond to the color reaction with m-dinitrobenzene.
已描述了一种定量测定“总17 - 羟基皮质类固醇”的可靠方法。尿液用硼氢化钠进行还原，硼氢化钠可将17 - 羟基 - 20 - 酮 - 21 - 脱氧类固醇还原为17,20 - 二羟基 - 21 - 脱氧类固醇，将17 - 酮类固醇还原为C19 17 - 羟基类固醇。随后用铋酸钠进行氧化，将所有C21 17 - 羟基类固醇氧化为相应的17 - 酮类固醇，以硫酸铵作为盐析剂，用氯仿∶丁醇进行萃取，最后用间二硝基苯进行显色反应并测量吸光度。尿液中原本存在的17 - 酮类固醇不会产生干扰，因为硼氢化钠会将它们还原为C19 17 - 羟基类固醇，而这些类固醇不能被铋酸钠氧化，也不会与间二硝基苯发生显色反应。
