Nordlind K, Thyberg J
Int Arch Allergy Appl Immunol. 1983;70(1):19-24. doi: 10.1159/000233267.
In the present investigation we studied the effects of different metal allergens on cell counts, dye exclusion, and fine structure of unsensitized guinea pig thymocytes cultured in vitro for up to 96 H. At concentrations which inhibit DNA synthesis of lymphoid cells, the metal salts most often caused cell degeneration as judged by dye exclusion test and electron microscopy. DNA synthesis stimulating concentrations of mercuric chloride gave an increase in the percentage of viable cells. Absolute cell counts were not changed with either DNA synthesis inhibiting or stimulating concentrations. The importance of using different methods in investigating effects of metal allergens on lymphocytes in vitro is pointed out.
在本研究中，我们研究了不同金属变应原对体外培养长达96小时的未致敏豚鼠胸腺细胞的细胞计数、染料排斥及精细结构的影响。在抑制淋巴细胞DNA合成的浓度下，根据染料排斥试验和电子显微镜判断，金属盐最常导致细胞变性。氯化汞的DNA合成刺激浓度使活细胞百分比增加。无论DNA合成抑制浓度还是刺激浓度，绝对细胞计数均未改变。文中指出了在体外研究金属变应原对淋巴细胞影响时使用不同方法的重要性。
