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粗糙脉孢菌18S - rRNA 3'端的核苷酸序列及其与5S - rRNA相互作用的研究

The nucleotide sequence at the 3'-end of Neurospora crassa 18S-rRNA and studies on the interaction with 5S-rRNA.

作者信息

Kelly J M, Cox R A

出版信息

Nucleic Acids Res. 1982 Nov 11;10(21):6733-45. doi: 10.1093/nar/10.21.6733.

DOI:10.1093/nar/10.21.6733
PMID:6217449
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326960/
Abstract

The sequence of more than 100 nucleotides at the 3'-end of Neurospora crassa 18S-rRNA was determined by chemical sequencing techniques. Extensive homologies with 18S-rRNA from other eukaryotes were found. Inspection of the nucleotide sequence at the 3'-end of N. crassa 5S-rRNA revealed the presence of sequences complementary to a region near the 3'-terminus of 18S-rRNA. Under the appropriate conditions a complex was formed between 18S-rRNA and 5S-rRNA (Tm 53 degrees C). Interaction was detected between 5S-rRNA and a specific 3'-terminal fragment from 18S-rRNA and between 18S-rRNA and a specific 3'-terminal fragment from 5S-rRNA. These findings are consistent with the idea that intermolecular base-pairing between nucleotides at the 3'-ends of 18S-rRNA and 5S-rRNA may be functionally important within the ribosome. Further investigation revealed that this intermolecular base-pairing is not essential for ribosome stability.

摘要

利用化学测序技术测定了粗糙脉孢菌18S - rRNA 3'端100多个核苷酸的序列。发现其与其他真核生物的18S - rRNA有广泛的同源性。对粗糙脉孢菌5S - rRNA 3'端核苷酸序列的检查显示，存在与18S - rRNA 3'末端附近区域互补的序列。在适当条件下，18S - rRNA与5S - rRNA之间形成了复合物（解链温度为53℃）。检测到5S - rRNA与18S - rRNA的特定3'末端片段之间以及18S - rRNA与5S - rRNA的特定3'末端片段之间存在相互作用。这些发现与以下观点一致，即18S - rRNA和5S - rRNA 3'端核苷酸之间的分子间碱基配对在核糖体中可能具有重要功能。进一步研究表明，这种分子间碱基配对对于核糖体稳定性并非必不可少。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd89/326960/5b136a796566/nar00390-0196-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd89/326960/0f69ab7f37ec/nar00390-0190-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd89/326960/18c7a87d23fb/nar00390-0193-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd89/326960/eddde76fad54/nar00390-0194-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd89/326960/5b136a796566/nar00390-0196-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd89/326960/0f69ab7f37ec/nar00390-0190-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd89/326960/18c7a87d23fb/nar00390-0193-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd89/326960/eddde76fad54/nar00390-0194-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd89/326960/5b136a796566/nar00390-0196-a.jpg

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Structure and function of ribosomal RNA.

本文引用的文献

1
The 3'-terminal primary structure of five eukaryotic 18S rRNAs determined by the direct chemical method of sequencing. The highly conserved sequences include an invariant region complementary to eukaryotic 5S rRNA.通过直接化学测序法确定的五种真核生物18S rRNA的3'末端一级结构。高度保守的序列包括一个与真核生物5S rRNA互补的恒定区。
Nucleic Acids Res. 1980 Oct 10;8(19):4365-76. doi: 10.1093/nar/8.19.4365.
2
3'-terminal nucleotide sequence of Crithidia fasciculata small ribosomal subunit RNA.纤细短膜虫小核糖体亚基RNA的3'末端核苷酸序列
FEBS Lett. 1981 Jun 15;128(2):298-304. doi: 10.1016/0014-5793(81)80103-2.
3
Nucleotide sequence of Xenopus laevis 18S ribosomal RNA inferred from gene sequence.
核糖体RNA的结构与功能。
Biochem J. 1985 Jul 1;229(1):1-17. doi: 10.1042/bj2290001.
4
Complementarity of sequences in low molecular weight RNAs to regions of messenger and ribosomal RNAs.低分子量RNA中序列与信使RNA和核糖体RNA区域的互补性。
Nucleic Acids Res. 1986 Jul 25;14(14):5741-60. doi: 10.1093/nar/14.14.5741.
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A low-molecular-weight RNA from mouse ascites cells that hybridizes to both 18S rRNA and mRNA sequences.一种来自小鼠腹水细胞的低分子量RNA，它能与18S核糖体RNA和信使RNA序列杂交。
Proc Natl Acad Sci U S A. 1986 Oct;83(19):7261-5. doi: 10.1073/pnas.83.19.7261.
从基因序列推断出的非洲爪蟾18S核糖体RNA的核苷酸序列。
Nature. 1981 May 21;291(5812):205-8. doi: 10.1038/291205a0.
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7
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