用于检测葡萄球菌抗原的酶联免疫吸附测定中葡萄球菌蛋白A的相互作用。
Interaction of staphylococcal protein A in enzyme-linked immunosorbent assays for detecting staphylococcal antigens.
作者信息
Notermans S, Timmermans P, Nagel J
出版信息
J Immunol Methods. 1982 Nov 26;55(1):35-41. doi: 10.1016/0022-1759(82)90074-6.
ELISA procedures for detecting staphylococcal antigens may be subject to interference by reactions between staphylococcal protein A (SPA) and IgG molecules. It was found that rabbit IgG reacted with SPA, both in the native state and after conjugation with peroxidase. Sheep IgG, however, did not react with SPA if conjugated with peroxidase. Peroxidase conjugated SPA reacted with rabbit IgG but not with sheep IgG. These results demonstrate that the source of IgG used in an ELISA system is of major importance to correct quantitation of staphylococcal antigens.
用于检测葡萄球菌抗原的酶联免疫吸附测定（ELISA）程序可能会受到葡萄球菌蛋白A（SPA）与IgG分子之间反应的干扰。研究发现，兔IgG无论处于天然状态还是与过氧化物酶结合后，均能与SPA发生反应。然而，羊IgG在与过氧化物酶结合后则不与SPA发生反应。与过氧化物酶结合的SPA能与兔IgG发生反应，但不与羊IgG发生反应。这些结果表明，ELISA系统中所用IgG的来源对于准确定量葡萄球菌抗原至关重要。
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