Fey H, Pfister H, Rüegg O
J Clin Microbiol. 1984 Jan;19(1):34-8. doi: 10.1128/jcm.19.1.34-38.1984.
We compared four versions of the enzyme-linked immunosorbent assay for their suitability for detecting staphylococcal enterotoxins. The sandwich with labeled antibody proved to be the best. We used it with a sorbent consisting of antibody-coated polystyrene spheres reacted with 20 ml of food extract. The sensitivity of the test was 0.1 ng of enterotoxin per ml, which is far below clinical relevance. The succinimidyl-pyridyl-dithio-propionate enzyme coupling method of Pharmacia was superior to the two-step glutaraldehyde technique. Interfering protein A was eliminated by the simple addition of normal rabbit serum to the extracts. A diagnostic kit is now available.
我们比较了四种酶联免疫吸附测定法检测葡萄球菌肠毒素的适用性。结果表明,标记抗体夹心测定法是最佳方法。我们将其与一种吸附剂一起使用,该吸附剂由包被抗体的聚苯乙烯球与20毫升食物提取物反应组成。该检测方法的灵敏度为每毫升0.1纳克肠毒素,远低于临床相关水平。Pharmacia公司的琥珀酰亚胺基吡啶二硫代丙酸酯酶偶联法优于两步戊二醛技术。通过向提取物中简单添加正常兔血清可消除干扰性蛋白A。现在已有诊断试剂盒。