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1
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2
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3
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5
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Enzymic synthesis of indole-3-acetyl-1-O-beta-d-glucose. II. Metabolic characteristics of the enzyme.吲哚 - 3 - 乙酰 - 1 - O - β - D - 葡萄糖的酶促合成。II. 该酶的代谢特性
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8
Enzymic synthesis of indole-3-acetyl-1-O-beta-d-glucose. I. Partial purification and characterization of the enzyme from Zea mays.吲哚 - 3 - 乙酰 - 1 - O - β - D - 葡萄糖的酶促合成。I. 玉米中该酶的部分纯化及特性研究
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本文引用的文献

1
Myo-Inositol Esters of Indole-3-acetic Acid as Seed Auxin Precursors of Zea mays L.吲哚 - 3 - 乙酸的肌醇酯作为玉米的种子生长素前体
Plant Physiol. 1980 Mar;65(3):422-7. doi: 10.1104/pp.65.3.422.
2
Concentration and Metabolic Turnover of Indoles in Germinating Kernels of Zea mays L.玉米发芽种子中吲哚的浓度和代谢周转率
Plant Physiol. 1980 Mar;65(3):415-21. doi: 10.1104/pp.65.3.415.
3
Concentration of Indole-3-acetic Acid and Its Derivatives in Plants.植物中吲哚-3-乙酸及其衍生物的浓度。
Plant Physiol. 1977 Aug;60(2):211-3. doi: 10.1104/pp.60.2.211.
4
Enzymatic Esterification of Indole-3-acetic Acid to myo-Inositol and Glucose.酶促酯化吲哚-3-乙酸到肌醇和葡萄糖。
Plant Physiol. 1974 Dec;54(6):846-51. doi: 10.1104/pp.54.6.846.
5
Concentrations of Indole-3-acetic Acid and Its Esters in Avena and Zea.燕麦和玉米中吲哚 - 3 - 乙酸及其酯类的浓度。
Plant Physiol. 1974 Sep;54(3):257-62. doi: 10.1104/pp.54.3.257.
6
Purification and Partial Characterization of a Glucan Containing Indole-3-acetic Acid.含吲哚-3-乙酸葡聚糖的纯化和部分特性分析。
Plant Physiol. 1972 Jul;50(1):176-82. doi: 10.1104/pp.50.1.176.
7
Gas-Liquid Chromatographic Analysis of Indole-3-acetic Acid Myoinositol Esters in Maize Kernels.玉米胚乳中吲哚-3-乙酸肌醇酯的气液色谱分析。
Plant Physiol. 1970 Nov;46(5):715-9. doi: 10.1104/pp.46.5.715.
8
A Quantitative Estimation of Alkali-labile Indole-3-Acetic Acid Compounds in Dormant and Germinating Maize Kernels.休眠和萌发玉米籽粒中碱不稳定吲哚-3-乙酸化合物的定量估计
Plant Physiol. 1969 Aug;44(8):1175-81. doi: 10.1104/pp.44.8.1175.
9
The Formation of Indoleacetylaspartic Acid in Pea Seedlings.豌豆幼苗中吲哚乙酰天冬氨酸的形成。
Plant Physiol. 1955 Jul;30(4):380-2. doi: 10.1104/pp.30.4.380.
10
I-(Indole-3-acetyl)-beta-D-glucose, a new compound in the metabolism of indole-3-acetic acid in plants.I-(吲哚-3-乙酰基)-β-D-葡萄糖,植物中吲哚-3-乙酸代谢中的一种新化合物。
Nature. 1961 Jul 29;191:493-4. doi: 10.1038/191493a0.

1-O-吲哚-3-基乙酰基-β-D-葡萄糖和吲哚-3-基乙酰基-肌醇的酶促合成

Enzymic synthesis of 1-O-indol-3-ylacetyl-beta-D-glucose and indol-3-ylacetyl-myo-inositol.

作者信息

Michalczuk L, Bandurski R S

出版信息

Biochem J. 1982 Nov 1;207(2):273-81. doi: 10.1042/bj2070273.

DOI:10.1042/bj2070273
PMID:6218801
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1153857/
Abstract

An enzyme fraction from extracts of immature kernels of Zea mays catalyses the formation of 1-O-indol-3-ylacetyl-beta-D-glucose from indol-3-ylacetic acid and UDP-glucose. A second enzyme fraction catalyses the formation of indol-3-ylacetyl-myo-inositol from 1-O-indol-3-ylacetyl-beta-D-glucose and myo-inositol. To our knowledge, this is the first example of hydroxy-group acylation by a 1-O-acyl sugar. The following reaction sequence is proposed: Indol-3-ylacetic acid + UDP-glucose leads to indol-3-ylacetylglucose + UDP (1) Indol-3-ylacetylglucose + myo-inositol leads to indol-3-ylacetyl-myo-inositol + glucose (2) The enzyme catalysing reaction (1) is called UDP-glucose:indol-3-ylacetate glucosyl-transferase (indol-3-ylacetylglucose synthase), and that catalysing reaction (2) is indol-3-ylacetylglucose:myo-inositol indol-3-ylacetyltransferase (indol-3-ylacetyl-myo-inositol synthase). We further show that indol-3-ylacetylglucose synthase is specific for UDP-glucose and, at the stage of purity tested, the enzyme will use either indol-3-ylacetic acid or naphthalene-1-acetic acid, but not 2.4-dichlorophenoxyacetic acid, as glucose acceptor. The indol-3-ylacetyl-myo-inositol synthase is specific for indol-3-ylacetyl-glucose and will not use naphthalene-1-acetylglucose as substrate, and it is specific for myo-inositol among the alcohol acceptors tested. Thus, of the auxins tested, only indol-3-ylacetic acid forms the myo-inositol ester.

摘要

来自玉米未成熟籽粒提取物的一种酶组分催化由吲哚 - 3 - 乙酸和UDP - 葡萄糖形成1 - O - 吲哚 - 3 - 基乙酰基 - β - D - 葡萄糖。第二种酶组分催化由1 - O - 吲哚 - 3 - 基乙酰基 - β - D - 葡萄糖和肌醇形成吲哚 - 3 - 基乙酰基 - 肌醇。据我们所知,这是1 - O - 酰基糖进行羟基酰化的首个实例。提出了以下反应序列:吲哚 - 3 - 乙酸 + UDP - 葡萄糖 → 吲哚 - 3 - 基乙酰葡萄糖 + UDP(1)吲哚 - 3 - 基乙酰葡萄糖 + 肌醇 → 吲哚 - 3 - 基乙酰基 - 肌醇 + 葡萄糖(2)催化反应(1)的酶称为UDP - 葡萄糖:吲哚 - 3 - 乙酸葡萄糖基转移酶(吲哚 - 3 - 基乙酰葡萄糖合酶),催化反应(2)的酶是吲哚 - 3 - 基乙酰葡萄糖:肌醇吲哚 - 3 - 基乙酰转移酶(吲哚 - 3 - 基乙酰基 - 肌醇合酶)。我们进一步表明,吲哚 - 3 - 基乙酰葡萄糖合酶对UDP - 葡萄糖具有特异性,并且在测试的纯度阶段,该酶将使用吲哚 - 3 - 乙酸或萘 - 1 - 乙酸作为葡萄糖受体,但不使用2,4 - 二氯苯氧乙酸。吲哚 - 3 - 基乙酰基 - 肌醇合酶对吲哚 - 3 - 基乙酰 - 葡萄糖具有特异性,不会使用萘 - 1 - 乙酰葡萄糖作为底物,并且在测试的醇类受体中对肌醇具有特异性。因此,在所测试的生长素中,只有吲哚 - 3 - 乙酸形成肌醇酯。