果蝇染色体的复制。IX. 保幼激素对体外多线染色体复制周期起始的刺激作用。
Replication of Drosophila chromosomes. IX. Stimulation of initiation of polytene replication cycles in vitro by juvenile hormone.
作者信息
Sinha P, Lakhotia S C
出版信息
Cell Differ. 1983 Jan;12(1):11-7. doi: 10.1016/0045-6039(83)90112-4.
A greater proportion of polytene nuclei show [3H]thymidine incorporation when third instar larval salivary glands of Drosophila nasuta are pulse-labelled after in vitro culture (3-24 h) in the presence of a juvenile hormone mimic, ZR 515. In glands chronically labelled with [3H]thymidine in the presence of ZR 515, more nuclei are seen to have entered new polytene replication cycles. Similarly, when salivary glands from larvae fed on 5-fluorodeoxyuridine to block polytene replication cycles at intersynthetic periods were cultured in vitro, new polytene replication cycles were initiated more quickly in the presence of ZR 515. These results suggest a stimulatory effect of juvenile hormone on new polytene replication cycles.
当在保幼激素类似物ZR 515存在的情况下对体外培养（3 - 24小时）的纳氏果蝇三龄幼虫唾液腺进行脉冲标记时，更大比例的多线核显示出[3H]胸腺嘧啶核苷掺入。在ZR 515存在的情况下用[3H]胸腺嘧啶核苷进行长期标记的腺体中，可以看到更多的核进入了新的多线复制周期。同样，当用5 - 氟脱氧尿苷喂养幼虫以在合成间期阻断多线复制周期的唾液腺在体外培养时，在ZR 515存在的情况下新的多线复制周期启动得更快。这些结果表明保幼激素对新的多线复制周期有刺激作用。
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