Amabis D C, Amabis J M
Dev Biol. 1984 Mar;102(1):1-9. doi: 10.1016/0012-1606(84)90169-6.
Injection of ecdysterone into mid-fourth instar larvae of Trichosia pubescens brought about drastic changes in the puffing pattern of the chromosomes of the salivary gland. The puff sequence induced by the hormone is quite similar to that which normally occurs at the end of larval life. Three classes of puffs were observed: the early induced, the late induced, and those repressed by the hormone. Among the late-induced puffs, several were DNA puffs. The protein synthesis inhibition prevents the induction of all late puffs and the process of gene amplification but does not affect the induction of the early puffs.
向毛颤蚓四龄中期幼虫注射蜕皮甾酮会使唾液腺染色体的胀泡模式发生剧烈变化。该激素诱导的胀泡序列与幼虫生命末期正常出现的序列非常相似。观察到三类胀泡：早期诱导的、晚期诱导的以及受该激素抑制的。在晚期诱导的胀泡中，有几个是DNA胀泡。蛋白质合成抑制会阻止所有晚期胀泡的诱导以及基因扩增过程，但不影响早期胀泡的诱导。
