Malicka-Blaszkiewicz M, Roth J S
FEBS Lett. 1983 Mar 7;153(1):235-9. doi: 10.1016/0014-5793(83)80155-0.
L1210 leukemia cell cytosol was analysed for the presence of DNase I activity. No free activity was determined in crude cytosol. DNase I enzyme was found to occur in a latent form bound to cytoplasmic actin. DNase-actin complex was partially isolated by Sephadex filtration and DNase I-like activity was demonstrated after SDS gel electrophoresis of the complex and enzyme renaturation. The results were compared with those for synthetic complex of pancreatic bovine DNase I and chicken muscle actin.
对L1210白血病细胞胞质溶胶进行了脱氧核糖核酸酶I（DNase I）活性分析。在粗制胞质溶胶中未检测到游离活性。发现DNase I酶以与细胞质肌动蛋白结合的潜伏形式存在。通过葡聚糖凝胶过滤部分分离了DNase-肌动蛋白复合物，并在对该复合物进行十二烷基硫酸钠（SDS）凝胶电泳和酶复性后证明了类似DNase I的活性。将结果与牛胰腺DNase I和鸡肌肉肌动蛋白的合成复合物的结果进行了比较。
